Chromatin mapping identifies BasR, a key regulator of bacteria-triggered production of fungal secondary metabolites.
Chromatin mapping identifies BasR, a key regulator of bacteria-triggered production of fungal secondary metabolites.
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DOI：
10.7554/elife.40969
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发表时间：
2018-10-12
期刊：
影响因子：
7.7
通讯作者：
Brakhage AA
中科院分区：
文献类型：
--
作者：
Fischer J;Müller SY;Netzker T;Jäger N;Gacek-Matthews A;Scherlach K;Stroe MC;García-Altares M;Pezzini F;Schoeler H;Reichelt M;Gershenzon J;Krespach MK;Shelest E;Schroeckh V;Valiante V;Heinzel T;Hertweck C;Strauss J;Brakhage AA
真核生物的表观遗传机制可以被细菌修饰，以在真核生物与微生物相互作用期间重新编程真核生物的反应。我们发现，细菌雷帕霉素链霉菌引发了染色质乙酰化的增加，从而激活了真菌构巢曲霉中沉默的次级代谢或基因簇。使用这个模型，我们的目标是了解基于细菌触发的染色质修饰的微生物通讯机制。利用乙酰化组蛋白H3的全基因组ChIP-seq分析，我们发现了A. nidulans与S.雷帕霉素和相关的乙酰化的变化，在真菌转录组。在参与次级代谢、氨基酸和氮代谢、信号传导和编码转录因子的基因中检测到不同乙酰化的组蛋白。进一步的分子分析确定了Myb样转录因子BasR作为真菌中细菌信号转导的调控节点，并显示其功能在其他曲霉属物种中是保守的。
The eukaryotic epigenetic machinery can be modified by bacteria to reprogram the response of eukaryotes during their interaction with microorganisms. We discovered that the bacterium Streptomyces rapamycinicus triggered increased chromatin acetylation and thus activation of the silent secondary metabolism ors gene cluster in the fungus Aspergillus nidulans. Using this model, we aim understanding mechanisms of microbial communication based on bacteria-triggered chromatin modification. Using genome-wide ChIP-seq analysis of acetylated histone H3, we uncovered the unique chromatin landscape in A. nidulans upon co-cultivation with S. rapamycinicus and relate changes in the acetylation to that in the fungal transcriptome. Differentially acetylated histones were detected in genes involved in secondary metabolism, in amino acid and nitrogen metabolism, in signaling, and encoding transcription factors. Further molecular analyses identified the Myb-like transcription factor BasR as the regulatory node for transduction of the bacterial signal in the fungus and show its function is conserved in other Aspergillus species.