Differential reactivity of putative genotype 2 hepatitis C virus F protein between chronic and recovered infections
Differential reactivity of putative genotype 2 hepatitis C virus F protein between chronic and recovered infections
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DOI：
10.1099/vir.0.83677-0
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发表时间：
2008-08-01
影响因子：
3.8
通讯作者：
Allain, Jean-Pierre
中科院分区：
文献类型：
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作者：
Chuang, Wing Chia-Ming;Allain, Jean-Pierre
迄今为止，所有关于丙型肝炎病毒（HCV）F蛋白的研究都是基于源自基因型1a或1b序列而非其他基因型的重组蛋白或单克隆抗体的体外表达。本研究的目的是制备假定的基因型 2 重组 F 蛋白，并评估其在慢性 HCV 感染或已从感染中恢复的个体血浆中的反应性。选择一种基因型 2 菌株用于细菌培养物中的 F 蛋白 (F-2) 和核心表达。开发了 ELISA 并将其应用于各种基因型的慢性感染或恢复感染患者的样本。 117 个样本中的抗 F-2 反应显示，慢性病患者的反应性明显高于康复的 HCV 感染献血者（P
To date, all studies regarding hepatitis C virus (HCV) F protein have been based on expression in vitrolin vivo of recombinant protein or monoclonal antibodies derived from genotype 1 a or 1 b sequences, but not from other genotypes. The objective of this study was to prepare a putative genotype 2 recombinant F protein and evaluate its reactivity in plasma from individuals with chronic HCV infection or who had recovered from infection. One genotype 2 strain was selected for F protein (F-2) and core expression in bacterial culture. An ELISA was developed and applied to samples from patients with chronic infection or recovered infection of various genotypes. The anti-F-2 response in 117 samples showed a significantly higher reactivity in chronic than in recovered HCV-infected blood donors (P