Self-Powered Temperature Sensor with Seebeck Effect Transduction for Photothermal-Thermoelectric Coupled Immunoassay
Self-Powered Temperature Sensor with Seebeck Effect Transduction for Photothermal-Thermoelectric Coupled Immunoassay
复制标题
用于光热-热电耦合免疫分析的具有塞贝克效应传导的自供电温度传感器
DOI：
10.1021/acs.analchem.9b05218
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发表时间：
2020-02-04
影响因子：
7.4
通讯作者：
Tang, Dianping
中科院分区：
文献类型：
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作者：
Huang, Lingting;Chen, Jialun;Tang, Dianping
基于Seebeck效应转导的自动温度传感器设计用于α-五蛋白蛋白（AFP）的光热疗法耦合的免疫测定。在该系统中，首先通过目标诱导的三明治型免疫反应捕获葡萄糖氧化酶（GOX）偶联的检测抗体。此后，通过GOX-葡萄糖系统氧化3,3'，S，S'-tetrametylbenzidine（TMB），产生的AS烯二氢过氧化氢（TMB）氧化为光热产物氧化TMB（OX-TMB）。在近红外（NIR）激光照射下，在3D打印的集成检测装置中，柔性热电模块在电信号中读出OX-TMB的温度变化。在最佳条件下，光热 - 心理耦合的免疫测定显示在0.5至60 ng mL（-1）的动态线性范围内检测到0.39 ng ml（-1）AFP。令人印象深刻的是，本文提出的这种策略为在超敏感生物传感器中应用许多其他高效热电材料提供了巨大的潜力。
A self-powered temperature sensor based on Seebeck effect transduction was designed for photothermal-thermoelectric coupled immunoassay of alpha-fetoprotein (AFP). In this system, glucose oxidase (GOx)-conjugated detection antibody was first captured onto the microplate by target-induced sandwich-type immunoreaction. Thereafter, the as-generated hydrogen peroxide via the GOx-glucose system oxidized 3,3',S,S'-tetrametylbenzidine (TMB) into photothermal product oxidized TMB (ox-TMB). Under near-infrared (NIR) laser irradiation, the temperature change of ox-TMB was read out in an electrical signal by the flexible thermoelectric module in a 3D-printed integrated detection device. Under optimal conditions, the photothermal-thermoelectric coupled immunoassay exhibited a limit of detection of 0.39 ng mL(-1) AFP over a dynamic linear range from 0.5 to 60 ng mL(-1). Impressively, such a strategy presented herein offers tremendous potentials for applying many other high-efficiency thermoelectric materials in ultrasensitive biosensors.