Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA)
Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA)
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DOI：
10.1007/s10592-007-9391-2
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发表时间：
2008-06-01
影响因子：
2.2
通讯作者：
Chiang, Tzen-Yuh
中科院分区：
文献类型：
--
作者：
Hung, Cheng-Yu;Wang, Kuo-Hsiung;Chiang, Tzen-Yuh
报道了11对新的野生茶树微卫星引物。Fora formosensis Kitamura.对采集自野生茶树种群的24份样品，以及栽培茶树和日本锦鸡儿，对这些简单序列重复标记进行检测。等位基因数从4个到18个不等。预期杂合度(H-E)为0.687-0.946，观测杂合度(H-O)为0.042-0.792。由于杂合子缺失，所有的基因座都显著偏离了Hardy-Weinberg的预期，表明稀有物种的遗传多态显著丧失。在大多数基因座上发现了显著的LD。这些引物可能为了解野生茶树的人口学和种群结构提供一种工具。
We report 11 novel microsatellite primer pairs for the wild tea, Camellia sinensis (L.) O. Kuntze forma formosensis Kitamura. These simple sequence repeat markers were tested in 24 samples collected from wild tea populations, and in cultivars and C. japonica. The number of alleles ranged from 4 to18. The expected (H-E) and observed (H-O) heterozygosity were 0.687-0.946 and 0.042-0.792, respectively. All loci were significantly deviated from Hardy-Weinberg expectations due to the heterozygote deficiency, indicating a dramatic loss of genetic polymorphisms in the rare species. Significant LD was discovered in most loci. These primers may provide a tool for understanding demography and population structure in wild tea.