MHC-I-restricted presentation of HIV-1 virion antigens without viral replication
MHC-I-restricted presentation of HIV-1 virion antigens without viral replication
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DOI：
10.1038/85493
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发表时间：
2001-03-01
期刊：
影响因子：
82.9
通讯作者：
Schwartz, O
中科院分区：
文献类型：
--
作者：
Buseyne, F;Le Gall, S;Schwartz, O
树突状细胞和巨噬细胞可以加工细胞外抗原以供MHC-I分子呈递。这种外源性途径可能在人类病毒感染期间CD 8（+）细胞毒性T淋巴细胞的活化中起关键作用。我们在这里表明，来自传入病毒粒子的HIV-1表位通过外源性MHC-I途径在原代人树突状细胞，并在较低程度上在巨噬细胞，导致细胞毒性T淋巴细胞活化的病毒蛋白合成的情况下。外源性抗原呈递需要足够的病毒-受体相互作用以及病毒和细胞膜的融合。这些结果提供了新的见解如何抗HIV细胞毒性T淋巴细胞可以被激活，并具有抗HIV疫苗设计的影响。
Dendritic cells and macrophages can process extracellular antigens for presentation by MHC-I molecules. This exogenous pathway may have a crucial role in the activation of CD8(+) cytotoxic T lymphocytes during human viral infections. We show here that HIV-1 epitopes derived from incoming virions are presented through the exogenous MHC-I pathway in primary human dendritic cells, and to a lower extent in macrophages, leading to cytotoxic T-lymphocyte activation in the absence of viral protein synthesis. Exogenous antigen presentation required adequate virus-receptor interactions and fusion of viral and cellular membranes. These results provide new insights into how anti-HIV cytotoxic T lymphocytes can be activated and have implications for anti-HIV vaccine design.