RNA guanine quadruplex invasion by complementary and homologous PNA probes
RNA guanine quadruplex invasion by complementary and homologous PNA probes
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DOI：
10.1021/ja051102y
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发表时间：
2005-06-08
影响因子：
15
通讯作者：
Armitage, BA
中科院分区：
文献类型：
--
作者：
Marin, VL;Armitage, BA
鸟嘌呤四链体由于在转录和翻译水平上调控基因表达而受到越来越多的关注。本文描述了短肽核酸（PNA）探针破坏稳定RNA四链体并与其靶序列杂交的能力。在一种情况下，PNA探针与靶标互补，导致形成沃森-克里克碱基配对双链体。在第二种情况下，PNA探针与靶标同源并形成杂交四链体结构。如基于沃森-克里克配对所施加的约束所预期的，杂交双链体以1：1的化学计量形成。然而，由于短PNA与原始RNA四链体的两半杂交的能力，杂交四链体以PNA 2：RNA化学计量形成。
Guanine quadruplexes are gaining increasing attention due to their suspected roles in regulating gene expression at the transcriptional and translational levels. This paper describes the ability of short peptide nucleic acid (PNA) probes to disrupt a stable RNA quadruplex and hybridize to their target sequence. In one case, the PNA probe is complementary to the target, resulting in formation of a Watson−Crick base-paired duplex. In the second case, the PNA probe is homologous to the target and forms a hybrid quadruplex structure. The hybrid duplex is formed in a 1:1 stoichiometry, as expected based on the constraints imposed by Watson−Crick pairing. However, the hybrid quadruplex is formed in a PNA2:RNA stoichiometry, due to the ability of the short PNA to hybridize with both halves of the original RNA quadruplex.