Human K10 epithelial keratin is the most abundant protein in airborne dust of both occupied and unoccupied school rooms
Human K10 epithelial keratin is the most abundant protein in airborne dust of both occupied and unoccupied school rooms
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DOI：
10.1039/b714802j
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发表时间：
2008-01-01
影响因子：
--
通讯作者：
Salzberg, Deborah
中科院分区：
文献类型：
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作者：
Fox, Karen;Castanha, Elisangela;Salzberg, Deborah
此前，有研究表明，在学校，大颗粒(2微米)和相关的细菌细胞包膜标志物的水平随着职业的增加而大大增加；人们假设两者的来源都是脱落的人类皮肤。在目前验证这一假设的工作中，使用房间空气净化器从有人和没有人的学校房间收集空气中的粉尘(>；50-100毫克)，然后进行蛋白质组分析。从粉尘中提取蛋白质，用双向凝胶电泳法(2D GE)分离。用胰酶释放的多肽原位消化蛋白质斑点，然后用基质辅助激光解吸/去离子、飞行时间质谱仪(MALDI-TOF-MS)和串联质谱仪(MALDI-TOF-MS-MS)进行分析。在考马斯蓝染色的凝胶中，2D凝胶中通常有一个单一的斑点；串联质谱仪鉴定该蛋白为K10上皮角蛋白。这一结果从实验上证实了之前的轶事报道，即人的皮肤很容易脱落到空气中，并表明在被占用的房间中观察到的微生物标志物和大颗粒水平的增加也来自皮肤。
Previously it was demonstrated that the levels of large particles (> 2 micron) and associated bacterial cell envelope markers increase greatly on occupation in schools; it was hypothesized that the source of both was shed human skin. In the current work to test this hypothesis, room air cleaners were used to collect airborne dust (> 50-100 mg) from occupied and unoccupied school rooms which was then subjected to proteomic analysis. Proteins were extracted from the dust and separated using two dimensional gel electrophoresis (2D GE). In situ digestion of protein spots with trypsin released peptides, which were subsequently analyzed by matrix assisted laser desorption/deionization, time-of-flight mass spectrometry (MALDI-TOF-MS) and tandem mass spectrometry (MALDI-TOF-MS-MS). In Coomassie blue stained gels, a single spot generally dominated the 2D gels; this protein was identified by tandem mass spectrometry as K10 epithelial keratin. The results experimentally confirm previous anecdotal reports that human skin is readily shed into air and suggest that increased levels of microbial markers and large particles observed in occupied rooms are also derived from skin.