Cortical projection to the subventricular zone and its effect on adult neurogenesis in mice
Cortical projection to the subventricular zone and its effect on adult neurogenesis in mice
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DOI：
10.1016/j.neulet.2023.137101
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发表时间：
2023-01
影响因子：
2.5
通讯作者：
Rei Ota;Ryota Yoshida;Tetsuji Mori
中科院分区：
文献类型：
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作者：
Rei Ota;Ryota Yoshida;Tetsuji Mori
不同的脑区/核团将轴突投射到脑室下区(SVZ)，这是一种出生后的神经源性利基。在成年人中，神经发生是由神经活动控制的，通过神经递质。谷氨酸是一种主要的兴奋性神经递质，谷氨酸受体在SVZ细胞中表达。虽然大脑皮层是谷氨酸的主要来源，内侧皮质将轴突投射到毗邻SVZ的内侧纹状体，但皮质神经元是否在体内调节成年神经发生仍不清楚。首先，通过宫内电穿孔将表达DsRed的质粒载体导入内侧皮质，以分析轴突投射。在成体阶段，DsRed标记的轴突在SVZ的背外侧、纹状体和隔区以及它们与神经母细胞接触的地方被检测到。此外，皮质投射的成熟和SVZ的成熟在出生后阶段似乎是同步的。接下来，在双侧内侧皮质造成刺伤，以中断皮质对SVZ的输入。在损伤后17天，SVZ的细胞增殖和神经母细胞向嗅球的切向迁移没有受到明显影响。各实验组靠近SVZ的纹状体内均可见成簇的神经母细胞，但内侧皮质损伤组神经母细胞簇的数量和大小均明显大于其他实验组。这些神经母细胞簇具有切向迁移到嗅球的细胞的形态。这些结果表明，皮质对SVZ的输入抑制了神经母细胞的径向迁移，使其在体内与迁移路径汇合。
Various brain regions/nuclei project axons to the subventricular zone (SVZ), a postnatal neurogenic niche. In adults, neurogenesis is controlled by neuronal activity, via neurotransmitters. Glutamate is a major excitatory neurotransmitter, and glutamate receptors are expressed in SVZ cells. Although the cerebral cortex is a major source of glutamate and the medial cortex projects axons to the medial striatum next to the SVZ, it remains unclear whether cortical neurons regulate adult neurogenesisin vivo. First, to analyze axonal projection, plasmid vector expressing DsRed was introduced to the medial cortex byin uteroelectroporation. At the adult stage, DsRed-labeled axons were detected in the dorsolateral, striatal, and septal areas of the SVZ, and where they were in contact with neuroblasts. Furthermore, maturation of the cortical projection and the SVZ appeared to synchronize during postnatal stages. Next, stab injuries were made in the bilateral medial cortex to interrupt cortical input to the SVZ. At 17 days post-injury, cell proliferation in the SVZ and tangential migration of neuroblasts to the olfactory bulb were not significantly affected. There were clusters of neuroblasts in the striatum close to the SVZ in all experimental groups, but the number and size of neuroblast clusters were significantly larger in the medial cortex-injured group compared with the other experimental groups. These neuroblast clusters had a morphology of tangentially migrating cells to the olfactory bulb. These results suggest that cortical input to the SVZ inhibits the radial migration of neuroblasts to converge with the migration pathwayin vivo.