THE ROLE OF ANTIBODIES AGAINST ALPHA-TOXIN AND TEICHOIC-ACID IN THE DIAGNOSIS OF STAPHYLOCOCCAL INFECTIONS
THE ROLE OF ANTIBODIES AGAINST ALPHA-TOXIN AND TEICHOIC-ACID IN THE DIAGNOSIS OF STAPHYLOCOCCAL INFECTIONS
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DOI：
10.1007/bf01641071
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发表时间：
1983-01-01
期刊：
影响因子：
7.5
通讯作者：
MOLLBY, R
中科院分区：
文献类型：
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作者：
JULANDER, IG;GRANSTROM, M;MOLLBY, R
采用酶联免疫吸附法（ELISA）检测纯化α-毒素和磷壁酸制剂对金黄色葡萄球菌感染的IgG和IgM反应。在确定正常人群中的抗体后，为所有年龄组设置截止水平。α-毒素酶联免疫吸附试验的敏感性高于抗葡萄球菌素中和试验（ASTA）。发现用这两种抗原测定IgM抗体的诊断价值有限。27例心内皮炎患者中有21例（78%）抗体阳性，14例合并败血症患者中有11例（79%）抗体阳性，20例无合并败血症患者中有8例（40%）抗体阳性，22例慢性骨髓炎患者中有12例（54%）抗体阳性。ELISA检测血清中IgG对磷壁酸和α-毒素的反应有一定差异，两种检测方法的平行性能提高了血清诊断率。在四组中，阳性患者分别增加到89%、86%、65%和64%，诊断特异性为93%。在败血性葡萄球菌感染中，所有患者（28 / 28）都可以通过适当间隔的配对样本进行诊断。
An enzyme-linked immunosorbent assay (ELISA) was used with purified α-toxin and teichoic acid preparations to measure the IgG and IgM response inStaphylococcus aureusinfections. After determining antibodies in a normal population, cut-off levels were set for all age groups. ELISA with α-toxin was more sensitive than the antistaphylolysin neutralization test (ASTA). Determining IgM antibodies with the two antigens was found to be of limited diagnostic value. Positive IgG titers against α-toxin were found in 21 of 27 patients (78%) with endocarditis, 11 of 14 (79%) with complicated septicemia, eight of 20 (40%) with uncomplicated septicemia and in 12 of 22 (54%) with chronic osteomyelitis. The IgG responses to teichoic acid and α-toxin were somewhat different when measured by ELISA, and the parallel performance of the two assays resulted in improved serological diagnostics. The number of positive patients increased to 89%, 86%, 65% and 64%, respectively, in the four groups with a diagnostic specificity of 93%. In septicemic staphylococcal infections, the diagnosis could be established in all patients (28 of 28) with adequately spaced paired samples.