Expression of glia maturation factor beta after cryogenic brain injury
Expression of glia maturation factor beta after cryogenic brain injury
复制标题
DOI：
10.1016/j.molbrainres.2004.09.027
复制
发表时间：
2005-01-05
期刊：
影响因子：
--
通讯作者：
Asai, K
中科院分区：
文献类型：
--
作者：
Hotta, N;Aoyama, M;Asai, K
胶质细胞成熟因子（GMFB）是一种生长和分化因子，作用于神经元和胶质细胞。我们采用免疫组织化学、逆转录聚合酶链反应（RT-PCR）、Western blotting和酶免疫分析法研究低温脑损伤后56天GMFB的表达。免疫组织化学分析显示，损伤周围gfap阳性的星形胶质细胞表达GMFB蛋白，在损伤后14天达到峰值。假手术动物脑中GMFB免疫反应性弱星形细胞表达。低温损伤（CI）在7天后诱导损伤侧GMFB mRNA表达，在14天后达到最大值。Western blotting显示GMFB蛋白的诱导从损伤后第1天开始，一直持续到损伤后第14天。在酶免疫分析中，损伤侧脑组织提取物GMFB蛋白浓度在损伤后14天达到峰值，而血清中GMFB蛋白浓度在损伤后1天达到峰值。这些数据表明，皮层低温脑损伤后，损伤区周围星形胶质细胞中GMFB的表达增加。这些发现可能为脑损伤后病理状态下GMFB的功能提供新的见解。(C) 2004 Elsevier B.V.版权所有
Glia maturation factor beta (GMFB) was identified as a growth and differentiation factor acting on neurons as well as glia. We investigated the expression of GMFB during 56 days after cryogenic brain injury, using immunohistochemistry, reverse transcriptase polymerase chain reaction (RT-PCR), Western blotting and enzyme immunoassay. Immunohistochemical analysis demonstrated that the GFAP-positive astrocytes around the lesion expressed GMFB protein, peaking 14 days after injury. Weak astrocytic expression of GMFB immunoreactivity was seen in sham-operated animal brains. Cryogenic injury (CI) induced GMFB mRNA in the lesioned side after 7 days with a maximum at 14 days. Western blotting revealed the induction of GMFB protein starting I day after injury, and continuing until 14 days after injury. In the enzyme immunoassay, GMFB protein concentration peaked 14 days after injury in extracts from the injured side of the brain, whereas in serum it peaked I day after injury. These data indicate that the expression of GMFB increased in the astrocytes around the lesioned area after cortical cryogenic brain injury. These findings may provide new insight into GMFB function in pathological conditions following brain injury. (C) 2004 Elsevier B.V. All rights reserved.