Identification of RAPD markers linked to the Uvf-1 gene conferring hypersensitive resistance against rust (Uromyces viciae-fabae) in Vicia faba L.
Identification of RAPD markers linked to the Uvf-1 gene conferring hypersensitive resistance against rust (Uromyces viciae-fabae) in Vicia faba L.
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DOI：
10.1007/s00122-003-1254-8
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发表时间：
2003-07-01
影响因子：
5.4
通讯作者：
Torres, AM
中科院分区：
文献类型：
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作者：
Avila, CM;Sillero, JC;Torres, AM
采用群体分离分析方法，筛选与蚕豆2N52品系对蚕豆锈菌1号生理小种的抗性相关基因连锁的随机扩增多态DNA标记。通过对抗病品系2N52与感病品系VF-176杂交的F-2群体的分析，确定了抗性的单基因性质，并在F-2：3衍生家系中得到进一步证实。通过对55株分离的F-2植株进行抗性筛选，证实了这两个分子标记的连锁。将OPD13(736)、OPL18(1032)和OPI20(900)标记定位于1号生理小种(UVF-1)的抗性基因偶联期。未检测到OPI20(900)与UVF-1之间的重组子。另外两个标记OPP02(1172)和OPR07(930)分别与排斥期基因连锁，距离分别为9.9和11.5 cM。讨论了分子标记辅助选择在选育抗锈病基因蚕豆新品种中的应用。
Bulk segregant analysis was used to identify random amplified polymorphic DNA (RAPD) markers linked to a gene determining hypersensitive resistance in Vicia faba line 2N52 against race 1 of the rust fungus Uromyces viciae-fabae. The monogenic nature of the resistance was determined by analyzing the F-2 population from a cross between resistant line 2N52 and susceptible line VF-176, and further confirmed in the F-2:3-derived families. Linkage of the RAPD markers was confirmed by screening 55 F-2 plants segregating for resistance. Three RAPD markers (OPD13(736), OPL18(1032) and OPI20(900)) were mapped in coupling phase to the resistance gene for race 1 (Uvf-1). No recombinants between OPI20(900) and Uvf-1 were detected. Two additional markers (OPP02(1172) and OPR07(930)) were linked to the gene in repulsion phase at a distance of 9.9 and 11.5 cM, respectively. The application of marker-assisted selection to develop new faba bean varieties with rust resistance genes is discussed.