The human leukocyte antigen (HLA)-C-specific "activatory" or "inhibitory" natural killer cell receptors display highly homologous extracellular domains but differ in their transmembrane and intracytoplasmic portions.
The human leukocyte antigen (HLA)-C-specific "activatory" or "inhibitory" natural killer cell receptors display highly homologous extracellular domains but differ in their transmembrane and intracytoplasmic portions.
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DOI：
10.1084/jem.183.2.645
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发表时间：
1996-02-01
期刊：
影响因子：
--
通讯作者：
Moretta L
中科院分区：
文献类型：
--
作者：
Biassoni R;Cantoni C;Falco M;Verdiani S;Bottino C;Vitale M;Conte R;Poggi A;Moretta A;Moretta L
自然杀伤细胞表达对主要组织相容性复合体I类分子具有特异性的克隆分布受体。人类白细胞抗原（HLA）-C特异性受体已在分子水平上被鉴定和克隆。它们不仅作为抑制性（p58）受体存在，也作为活化性（p50）受体存在。在此我们表明，p50和p58在由两个免疫球蛋白样结构域形成的细胞外区域具有高度同源性。相比之下，它们的跨膜和胞质部分存在重大差异。p58具有一个含不寻常抗原受体活化基序的76 - 84个氨基酸的胞质尾，而p50的特征是一个较短的39个氨基酸的尾。此外，p58具有一个非极性跨膜部分，而p50含有带电荷的氨基酸赖氨酸。这些数据有力地表明，具有相同HLA - C等位基因特异性的受体由于其不同的跨膜/胞质部分能够介导相反作用的功能。
Natural killer cells express clonally distributed receptors specific for major histocompatibility complex class I molecules. The human leukocyte antigen (HLA)-C-specific receptors have been molecularly identified and cloned. They exist not only as inhibitory (p58) but also as activatory (p50) receptors. Here we show that p50 and p58 are highly homologous in their extracellular regions formed by two Ig-like domains. In contrast, major differences exist in their transmembrane and cytoplasmic portions. Whereas p 58 displays a 76-84-amino acid cytoplasmic tail containing an unusual antigen receptor activation motif, p50 is characterized by a shorter 39-amino acid tail. In addition, whereas p58 has a nonpolar transmembrane portion, p50 contains the charged amino acid Lys. These data strongly suggest that receptors with identical HLA-C allele specificity can mediate functions of opposite sign owing to their different transmembrane/cytoplasmic portions.