Detection and Cellular Tropism of Porcine Astrovirus Type 3 on Breeding Farms
Detection and Cellular Tropism of Porcine Astrovirus Type 3 on Breeding Farms
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DOI：
10.3390/v11111051
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发表时间：
2019-11-01
期刊：
影响因子：
4.7
通讯作者：
Arruda, Bailey L.
中科院分区：
文献类型：
--
作者：
Rawal, Gaurav;Ferreyra, Franco Matias;Arruda, Bailey L.
星状病毒在多种物种中引起疾病。然而，人们对大多数星状病毒的流行病学知之甚少，其中包括猪星状病毒 3 型 (PoAstV3)，它是猪脊髓灰质炎的假定原因。因此，我们对有或没有报道的 PoAstV3 相关神经系统疾病的母猪场进行了一项横断面研究，这些猪场的断奶仔猪。此外，还从一个农场方便地选择了仔猪子集进行总体和组织学评估。通过原位杂交评估了 PoAstV3 在肠溶系统中的分布。通过 RT-qPCR 在粪便样本中检测到的 PoAstV3 在所有农场 (65-85%) 的母猪和仔猪 (66-90%) 中频繁检测到。三个月后，PoAstV3 在一个农场以相似的检测频率（77% 与 85%）被检测到。根据循环定量值确定的病毒排出表明，仔猪比成年猪排出的病毒量更高。未发现胃肠道疾病与 PoAstV3 之间存在联系。然而，PoAstV3 在仔猪肌间神经丛神经元中原位检测到，阐明了病毒从胃肠道传播到中枢神经系统的可能途径。这些数据表明，PoAstV3 具有流行潜力，与母猪相比，哺乳仔猪在粪便中排出的数量更多，并且在检测到该病毒的农场中感染广泛。
Astroviruses cause disease in a variety of species. Yet, little is known about the epidemiology of a majority of astroviruses including porcine astrovirus type 3 (PoAstV3), which is a putative cause of polioencephalomyelitis in swine. Accordingly, a cross-sectional study was conducted on sow farms with or without reported PoAstV3-associated neurologic disease in growing pigs weaned from those farms. Additionally, a conveniently selected subset of piglets from one farm was selected for gross and histologic evaluation. The distribution of PoAstV3 in the enteric system was evaluated through in situ hybridization. PoAstV3, as detected by RT-qPCR on fecal samples, was frequently detected across sows and piglets (66-90%) on all farms (65-85%). PoAstV3 was detected subsequently at a similar detection frequency (77% vs 85%) on one farm after three months. Viral shedding, as determined by the cycle quantification value, suggests that piglets shed higher quantities of virus than adult swine. No link between gastrointestinal disease and PoAstV3 was found. However, PoAstV3 was detected by in situ in myenteric plexus neurons of piglets elucidating a possible route of spread of the virus from the gastrointestinal tract to the central nervous system. These data suggest PoAstV3 has endemic potential, is shed in the feces at greater quantities by suckling piglets when compared to sows, and infection is widespread on farms in which it is detected.