The yeast HPR1 gene has a functional role in transcriptional elongation that uncovers a novel source of genome instability
The yeast HPR1 gene has a functional role in transcriptional elongation that uncovers a novel source of genome instability
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DOI：
10.1101/gad.11.24.3459
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发表时间：
1997-12-15
影响因子：
10.5
通讯作者：
Aguilera, A
中科院分区：
文献类型：
--
作者：
Chávez, S;Aguilera, A
酵母HPR 1基因在基因组稳定性中起着重要作用，正如观察到的那样，hPR 1突变体具有高频率的DNA重复重组和染色体丢失。在这里，我们报告说，HPR 1是转录延伸所需的。由组成型和受调控的酵母启动子驱动的转录不能延伸通过hpr 1 Delta细胞中的细菌lacZ编码区，但通过其他序列如酵母PHO 5有效地进行。我们表明，HPR 1是不需要的转录激活和以前报道的影响hpr 1三角洲上的激活不同的启动子是一个后果的hpr 1三角洲细胞通过lacZ，作为报告延长转录的能力。在转录延伸抑制剂6-氮尿嘧啶存在下，在hpr 1 Delta细胞的酵母DNA序列中也观察到转录缺陷。在所有的情况下，在hpr 1 δ转录延伸的阻断与高频率的缺失和质粒不稳定性的增加，我们在这里报告。因此，除了识别参与转录延伸的新元素外，我们的工作还为基因组不稳定性的新来源提供了证据。
The yeast HPR1 gene plays an important role in genome stability, as indicated by the observation that hpr1 mutants have high frequencies of DNA repeat recombination and chromosome loss. Here we report that HPR1 is required for transcriptional elongation. Transcription driven from constitutive and regulated yeast promoters cannot elongate through the bacterial lacZ coding region in hpr1 Delta cells, but progresses efficiently through other sequences such as yeast PHO5. We show that HPR1 is not required for transcription activation and that the previously reported effects of hpr1 Delta on the activation of different promoters is a consequence of the incapacity of hpr1 Delta cells to elongate transcription through lacZ, used as reporter. Transcriptional defects are also observed in yeast DNA sequences of hpr1 Delta cells in the presence of the transcription elongation inhibitor 6-azauracil. In all cases, the blockage of transcription elongation in hpr1 Delta is associated with both the high frequency of deletions and the increase in plasmid instability that we report here. Therefore, in addition to the identification of a new element involved in transcriptional elongation, our work provides evidence for a new source of genomic instability.