Gamma irradiation can reduce muscle damage in mdx dystrophic mice
Gamma irradiation can reduce muscle damage in mdx dystrophic mice
复制标题
DOI：
10.1007/s004010050936
复制
发表时间：
1998-12-01
影响因子：
12.7
通讯作者：
Corsi, A
中科院分区：
文献类型：
--
作者：
Granata, AL;Vecchi, C;Corsi, A
我们报告了单次伽玛照射对16-20天龄正常和mdx小鼠一侧比目鱼肌的影响。在45、75和90日龄时，取肌肉中腹的冰冻切片进行显微镜检查。在正常小鼠中，纤维的生长在照射后明显减少，而没有纤维丢失。Mdx小鼠比目鱼肌照射后，再生的中心核纤维数量很少，纤维总数显着减少。据推测，自动物出生以来存活下来的外周有核纤维的数量几乎一直高于对侧未受辐射的肢体。众所周知，卫星细胞的增殖和融合对于纤维损伤后的再生和肌肉纤维的正常出生后的生长都是必需的：辐射似乎减少了再生和生长。这表明，辐射通过减少与生长相关的融合来减少损害。我们的假设间接地指出了营养不良蛋白缺乏和纤维坏死之间的显著联系，并很好地解释了自然和实验条件下小鼠营养不良的许多特征。
We report the effects of a single gamma irradiation delivered to the soleus muscle of one limb of normal and mdx mice at the age of 16-20 days. At 45, 75 and 90 days of age transverse cryostat sections from the mid-belly of the muscles were used for microscopic examination. In normal mice the growth of fibres was appreciably reduced by irradiation without fibre loss. In the irradiated soleus of mdx mice the number of the regenerated centrally nucleated fibres was very small and the total number of fibres was remarkably reduced. The number of the peripherally nucleated fibres, presumably surviving since the birth of the animal, was almost consistently larger than in the contralateral non-irradiated limb. The cross-sectional area of the irradiated fibres was smaller It is well known that proliferation and fusion of satellite cells are required both for regeneration after fibre damage and for the normal postnatal growth of muscle fibres: irradiation appears to reduce regeneration and growth. It is suggested that irradiation reduces damage by reducing fusion associated with growth. Our hypothesis indirectly indicates a significant link between dystrophin deficiency and fibre necrosis and accounts well for many features of mouse dystrophy under natural and experimental conditions.