Modeling Brain Vasculature Immune Interactions In Vitro
Modeling Brain Vasculature Immune Interactions In Vitro
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DOI：
10.1101/cshperspect.a041185
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发表时间：
2023-09-01
影响因子：
5.4
通讯作者：
Engelhardt, Britta
中科院分区：
文献类型：
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作者：
Lyck, Ruth;Nishihara, Hideaki;Engelhardt, Britta
内皮血脑屏障 (BBB) 通过严格控制血液和 CNS 之间分子和免疫细胞的运动，保护中枢神经系统 (CNS) 神经元免受血液环境变化的影响。免疫细胞跨血管壁迁移是一个多步骤过程，受内皮和免疫细胞上不同信号和粘附分子顺序相互作用的调节。考虑到其独特的屏障特性和运输分子表达谱，已经观察到免疫细胞跨血脑屏障迁移的特殊适应性。因此，血脑屏障的体外模型有助于探索免疫细胞跨血脑屏障运输的精确细胞和分子机制。需要克服的挑战是脑微血管内皮细胞的屏障特性不是固有的，并且很容易在培养物中丢失。我们重点关注人类体外 BBB 模型，在此讨论可用的 BBB 体外模型是否适用于探索跨 BBB 免疫细胞运输的具体机制。
The endothelial blood-brain barrier (BBB) protects central nervous system (CNS) neurons from the changeable milieu of the bloodstream by strictly controlling the movement of molecules and immune cells between the blood and the CNS. Immune cell migration across the vascular wall is a multistep process regulated by the sequential interaction of different signaling and adhesion molecules on the endothelium and the immune cells. Accounting for its unique barrier properties and trafficking molecule expression profile, particular adaptions in immune cell migration across the BBB have been observed. Thus, in vitro models of the BBB are desirable to explore the precise cellular and molecular mechanisms involved in immune cell trafficking across the BBB. The challenge to overcome is that barrier properties of brain microvascular endothelial cells are not intrinsic and readily lost in culture. With a focus on human in vitro BBB models, we here discuss the suitability of available in vitro models for the BBB for exploring the specific mechanisms involved in immune cell trafficking across the BBB.