Carbohydrate-restricted diet alters the gut microbiota, promotes senescence and shortens the life span in senescence-accelerated prone mice
Carbohydrate-restricted diet alters the gut microbiota, promotes senescence and shortens the life span in senescence-accelerated prone mice
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DOI：
10.1016/j.jnutbio.2019.108326
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发表时间：
2020-04-01
影响因子：
5.6
通讯作者：
Tsuduki, Tsuyoshi
中科院分区：
文献类型：
--
作者：
He, Chaoqi;Wu, Qiming;Tsuduki, Tsuyoshi
这项研究检查了碳水化合物限制饮食对衰老，脑功能，肠道细菌和寿命的影响，以确定长期碳水化合物限制对衰老加速倾向小鼠（SAMP 8）衰老过程的影响。三周龄的雄性SAMP 8在初步喂养一周后被分成三组。其中一组被给予控制饮食，而其他人分别喂高脂肪和碳水化合物限制饮食。每组中的小鼠进一步分为两个亚组，其中一个是寿命测量组。其他组自由进食，直到小鼠50周龄。在实验结束前，采用被动回避实验评价大鼠的学习记忆能力。试验期后，获得血清和各种小鼠器官并提交进行分析。与其他两个饮食组相比，碳水化合物限制饮食组的存活率显着降低。被动回避实验显示，与对照组相比，碳水化合物限制饮食组的学习和记忆能力显著下降。组织中脂质过氧化物含量的测定显示，与对照组和高脂组相比，限糖饮食组的脑和脾脏中脂质过氧化物含量显著增加。此外，在碳水化合物限制饮食组中观察到血清IL-6和IL-1 β水平（炎症指标）显著升高、肠杆菌减少（具有抗炎作用）、炎症诱导肠杆菌增加和盲肠中短链脂肪酸水平降低。因此，碳水化合物限制饮食被揭示为促进SAMP 8中的衰老和寿命缩短。(C)2019爱思唯尔公司All rights reserved.
This study examined the effects of a carbohydrate-restricted diet on aging, brain function, intestinal bacteria and the life span to determine long-term carbohydrate-restriction effects on the aging process in senescence-accelerated prone mice (SAMP8). Three-week-old male SAMP8 were divided into three groups after a week of preliminary feeding. One group was given a controlled diet, while the others fed on high-fat and carbohydrate-restricted diets, respectively. The mice in each group were further divided into two subgroups, of which one was the longevity measurement group. The other groups fed ad libitum until the mice were 50 weeks old. Before the test period termination, passive avoidance test evaluated the learning and memory abilities. Following the test period, serum and various mice organs were obtained and submitted for analysis. The carbohydrate-restricted diet group exhibited significant decrease in the survival rate as compared to the other two diet groups. The passive avoidance test revealed a remarkable decrease in the learning and memory ability of carbohydrate-restricted diet group as compared to the control-diet group. Measurement of lipid peroxide level in tissues displayed a marked increase in the brain and spleen of carbohydrate-restricted diet group than the control-diet and high-fat diet groups. Furthermore, notable serum IL-6 and IL-1 beta level (inflammation indicators) elevations, decrease in Enterobacteria (with anti-inflammatory action), increase in inflammation-inducing Enterobacteria and lowering of short-chain fatty acids levels in cecum were observed in the carbohydrate-restricted diet group. Hence, carbohydrate-restricted diet was revealed to promote aging and shortening of life in SAMP8. (C) 2019 Elsevier Inc. All rights reserved.