Dietary regulation of the activities of ornithine decarboxylase and S-adenosylmethionine decarboxylase in rats.
Dietary regulation of the activities of ornithine decarboxylase and S-adenosylmethionine decarboxylase in rats.
复制标题
大鼠鸟氨酸脱羧酶和S-腺苷甲硫氨酸脱羧酶活性的饮食调节。
DOI：
10.1093/jn/113.10.1927
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
M. Swendseid
中科院分区：
文献类型：
--
作者：
Patricia Moore;M. Swendseid
本研究旨在探讨多胺生物合成的两个关键酶--鸟氨酸脱羧酶（ODC）和S-腺苷甲硫氨酸脱羧酶（SAMDC）在大鼠组织中的饮食调节作用。通过胃管向禁食大鼠施用氨基酸混合物（模式化后的酪蛋白）、完全饮食或几种饮食组分。在特定的时间间隔，在12小时内杀死大鼠，ODC和SAMDC的活动进行了测量，在以下组织：肝，肾，小肠，脑和骨骼肌。氨基酸混合物，以及完整的饮食，引起快速和大幅增加的ODC和SAMDC活动在肝脏，肾脏和肠道。在大脑和肌肉中，SAMDC活性仅增加。放线菌素D的研究表明，SAMDC和ODC的活性在转录时受到调节。引起所观察到的酶活性增加的机制被假定为氨基酸流入细胞，而不是响应饮食刺激分泌的激素的影响。
The purpose of this study was to investigate the dietary regulation of the two key enzymes in polyamine biosynthesis, ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (SAMDC) in rat tissues. Either an amino acid mix (patterned after casein), a complete diet or several dietary components were administered to fasting rats by gastric tube. Rats were killed at specific time intervals, over a 12-hour period, and the activities of ODC and SAMDC were measured in the following tissues: liver, kidney, small intestine, brain and skeletal muscle. The amino acid mix, as well as the complete diet, elicited rapid and substantial increases in ODC and SAMDC activities in liver, kidney and intestine. In brain and muscle SAMDC activity only was increased. Studies with actinomycin D showed that SAMDC and ODC activities are regulated at transcription. The mechanism eliciting the observed increases in enzyme activities is postulated to be the influx of amino acids into cells rather than the influence of hormones secreted in response to dietary stimulation.