vavCre transgenic mice:: A tool for mutagenesis in hematopoietic and endothelial lineages
vavCre transgenic mice:: A tool for mutagenesis in hematopoietic and endothelial lineages
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DOI：
10.1002/gene.10161
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发表时间：
2002-12-01
期刊：
影响因子：
1.5
通讯作者：
Print, CG
中科院分区：
文献类型：
--
作者：
Georgiades, P;Ogilvy, S;Print, CG
Cre转基因小鼠可用于删除特定体细胞组织中loxP位点两侧的基因序列。我们已经产生了vavCre转基因小鼠，可用于灭活成人造血细胞和内皮细胞中的基因。在这些动物中，Cre转基因在小鼠vav基因调控元件的控制下表达。为了评估其有效性，将vavCre转基因小鼠与R26R小鼠杂交，R26R小鼠仅在发生cre介导重组的细胞中表达lacZ报告基因。采用-半乳糖苷酶组织化学和流式细胞术对VavCre/R26R双杂合子代进行分析。vavcre介导的重组发生在所有造血器官的大多数造血细胞中，包括造血祖细胞丰富的骨髓。重组也发生在大多数内皮细胞和生殖细胞中，但很少发生在其他类型的细胞中。造血和内皮谱系的重组可能部分反映了它们共同的个体发生，并为同时进行泛造血和内皮突变提供了独特的工具。(C) 2002 Wiley-Liss, Inc。
Cre transgenic mice can be used to delete gene sequences flanked by loxP sites in specific somatic tissues. We have generated vavCre transgenic mice, which can be used to inactivate genes specifically in adult hematopoietic and endothelial cells. In these animals, a Cre transgene is expressed under control of murine vav gene regulatory elements. To assess their usefulness, vavCre transgenic mice were bred with R26R mice, which express a lacZ reporter gene only in cells where Cre-mediated recombination has occurred. VavCre/R26R double-heterozygous offspring were analyzed by beta-galactosidase histochemistry and flow cytometry. VavCre-mediated recombination occurred in most hematopoietic cells of all hematopoietic organs, including the hematopoietic progenitor-rich bone marrow. Recombination also occurred in most endothelial and germ cells, but only rarely in other cell types. The recombination in both hematopoietic and endothelial lineages may partly reflect their putative shared ontogeny and provides a unique tool for simultaneous pan-hematopoietic and endothelial mutagenesis. (C) 2002 Wiley-Liss, Inc.