IL-27 regulates the adherence, proliferation, and migration of MSCs and enhances their regulatory effects on Th1 and Th2 subset generations.
IL-27 regulates the adherence, proliferation, and migration of MSCs and enhances their regulatory effects on Th1 and Th2 subset generations.
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IL-27 调节 MSC 的粘附、增殖和迁移，增强其对 Th1 和 Th2 亚群世代的调节作用
DOI：
10.1007/s12026-017-8929-8
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发表时间：
2017-08
影响因子：
4.4
通讯作者：
Luan X
中科院分区：
文献类型：
--
作者：
Xu F;Yi J;Wang Z;Hu Y;Han C;Xue Q;Zhang X;Luan X
白细胞介素27（IL-27）调节T细胞功能并参与炎症。已有研究表明，人胎盘间充质基质细胞（human placenta-derived mesenchymal stromal cells，hPMSCs）具有抑制T细胞反应和减轻炎症反应的作用。然而，目前尚不清楚IL-27是否可以调节hPMSC功能。在这里，我们研究了IL-27对hPMSCs的粘附、迁移和增殖的影响以及免疫调节作用。结果表明，hPMSCs表达IL-27受体α链（IL-27R α）。30 ng/ml的IL-27抑制hPMSC粘附和增殖，而20或30 ng/ml的IL-27促进hPMSC的迁移，如使用实时细胞分析（RTCA）所确定的。此外，IL-27通过增强人外周血中活化的T细胞的Th2和抑制Th1亚群的产生来促进hPMSC的调节作用。IL-27还通过Janus激酶（JAK）/信号转导子和转录激活子1（STAT1）信号传导途径增加hPMSC中程序性死亡配体1（PDL 1）的表达水平，增强hPMSC从CD4 + T细胞分泌IL-10的能力。总之，IL-27对hPMSC的粘附、增殖和迁移具有显著的调节作用。IL-27通过JAK/STAT1途径增加hPMSC中PDL 1的表达水平，从而增强hPMSC对Th1和Th2细胞生成以及CD4 + T细胞分泌IL-10的调节作用。
Interleukin 27 (IL-27) regulates T cell function and is involved in inflammation. It has been reported that human placenta-derived mesenchymal stromal cells (hPMSCs) can inhibit T cell responses and attenuate inflammation reactions. However, it is unclear whether IL-27 can regulate hPMSC function. Here, we examined the effects of IL-27 upon adherence, migration, and proliferation as well as the immunomodulatory effects of hPMSCs. The results show that IL-27 receptor α chain (IL-27Rα) is expressed in hPMSCs. IL-27 at 30 ng/ml inhibited hPMSC adherence and proliferation, while the migration of hPMSCs was promoted with IL-27 at doses of 20 or 30 ng/ml, as determined with use of real-time cell analysis (RTCA). Moreover, IL-27 promoted regulatory effects of hPMSCs through enhancing Th2 and suppressing Th1 subset generation from activated T cells in human peripheral blood. IL-27 also enhanced the ability of hPMSCs to secrete IL-10 from CD4+T cells through increased expression levels of the programmed death ligand 1 (PDL1) in hPMSCs via the Janus kinase (JAK)/signal transducer and activator of transcription 1 (STAT1) signaling pathway. In conclusion, IL-27 has significant modulatory effects on adherence, proliferation, and migration of hPMSCs. IL-27 increased PDL1 expression levels in hPMSCs via the JAK/STAT1 pathway, which then enhanced the regulatory effects of hPMSCs upon Th1 and Th2 cell generations and IL-10 secretion from CD4+T cells.
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影响因子：
4
作者：
Dowling CM;Herranz Ors C;Kiely PA
通讯作者：
Kiely PA
影响因子：
--
作者：
Di Carlo E;Sorrentino C;Zorzoli A;Di Meo S;Tupone MG;Ognio E;Mincione G;Airoldi I
通讯作者：
Airoldi I
间充质基质细胞来源的外泌体的免疫调节作用
影响因子：
4.4
作者：
Chen, Wancheng;Huang, Yukai;Xiao, Yang
通讯作者：
Xiao, Yang
影响因子：
4.4
作者：
Fitzgerald, Denise C.;Ciric, Bogoljub;Rostami, Abdolmohamad
通讯作者：
Rostami, Abdolmohamad
影响因子：
4.4
作者：
Villarino, AV;Larkin, J;Hunter, CA
通讯作者：
Hunter, CA