Molecular architecture of the bacterial tripartite multidrug efflux pump focusing on the adaptor bridging model
Molecular architecture of the bacterial tripartite multidrug efflux pump focusing on the adaptor bridging model
复制标题
DOI：
10.1007/s12275-015-5248-4
复制
发表时间：
2015-05
影响因子：
3
通讯作者：
Saemee Song;Jin-Sik Kim;Kangseok Lee;N. Ha
中科院分区：
文献类型：
--
作者：
Saemee Song;Jin-Sik Kim;Kangseok Lee;N. Ha
革兰氏阴性细菌通过由内膜转运蛋白、外膜通道蛋白和周质衔接蛋白组成的三方药物外排泵排出多种有毒物质。这些泵形成三方组件，可以跨越整个细胞膜，包括内膜和外膜。关于三方药物流出泵中各个组件的组装存在争议。最近的结构和功能研究加深了我们对泵的组装和工作机制的理解。在这里，我们根据最近的结构和功能研究重新评估装配模型。特别是，这项研究重点关注“适配器桥接模型”，强调了六聚体组装中外膜通道蛋白的尖端区域与周质适配器蛋白之间的啮合齿轮状相互作用。
Gram-negative bacteria expel a wide range of toxic substances through tripartite drug efflux pumps consisting of an inner membrane transporter, an outer membrane channel protein, and a periplasmic adaptor protein. These pumps form tripartite assemblies which can span the entire cell envelope, including the inner and outer membranes. There have been controversial findings regarding the assembly of the individual components in tripartite drug efflux pumps. Recent structural and functional studies have advanced our understanding of the assembly and working mechanisms of the pumps. Here, we re-evaluate the assembly models based on recent structural and functional studies. In particular, this study focuses on the ‘adaptor bridging model’, highlighting the intermeshing cogwheel-like interactions between the tip regions of the outer membrane channel protein and the periplasmic adaptor protein in the hexameric assembly.