Metabolic Co-operation between Biochemically Marked Mammalian Cells in Tissue Culture
Metabolic Co-operation between Biochemically Marked Mammalian Cells in Tissue Culture
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组织培养中生化标记的哺乳动物细胞之间的代谢合作
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通讯作者：
J. Pitts
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作者：
H. Subak;R. R. Burk;J. Pitts
将缺乏肌苷焦磷酸化酶活性的仓鼠成纤维细胞系BHK 21的遗传变异体（IPP~细胞）的细胞与具有肌苷焦磷酸化酶活性的BHK 21亚系细胞（IPP ~+细胞）混合培养。如果不与IPP +细胞接触，IPP+细胞不将添加的[ 3 H]次黄嘌呤掺入核酸中。JPP +细胞总是掺入[ 3 H]次黄嘌呤，当与IPP +细胞直接或间接接触时，IPP+细胞也掺入同位素。细胞与细胞的接触似乎是IPP细胞获得代谢功能所必需的。可能的分子基础和一般影响的现象进行了讨论。
SUMMARY Cells of a genetic variant of the hamster fibroblast line BHK 21 which lack inosinic pyrophosphorylase activity {IPP~ cells) and therefore cannot normally incorporate [ 3 H]hypo-xanthine were grown in mixed culture with cells of BHK 21 sublines which have inosinic pyrophosphorylase activity (IPP + cells). If not in contact with IPP + cells, 1PP~ cells do not incorporate added [ 3 H]hypoxanthine into nucleic acid. JPP + cells always do incorporate [ 3 H]hypoxanthine and IPP~ cells when in direct or indirect contact with IPP + cells also incorporate the isotope. Cell to cell contact appears to be essential for this gain of a metabolic function by IPP~ cells. The possible molecular basis and general implications of the phenomenon are discussed.