Effects of growth and exercise on composition, structural maturation and appearance of osteoarthritis in articular cartilage of hamsters
Effects of growth and exercise on composition, structural maturation and appearance of osteoarthritis in articular cartilage of hamsters
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DOI：
10.1111/j.1469-7580.2010.01270.x
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发表时间：
2010-09-01
影响因子：
2.4
通讯作者：
Helminen, Heikki J.
中科院分区：
文献类型：
--
作者：
Julkunen, Petro;Halmesmaki, Esa P.;Helminen, Heikki J.
关节软骨的组成和结构在负荷的影响下由软骨细胞维持和重塑。研究了运动诱导的生长和老化仓鼠关节软骨的组成、结构、力学性能和组织完整性的变化。从1、3、6、12和15月龄的仓鼠的近端胫骨收获关节软骨样品（n = 191）。仓鼠被分为跑步者和对照组。跑步者在1至3个月（跑步者组3个月、12个月和15个月大的仓鼠）或1至6个月（跑步者组6个月大的仓鼠）之间可以自由使用跑步轮。对照组动物采用久坐不动的生活方式。对软骨样品进行了机械压痕测试和深度方向的成分和结构分析。此外，使用组织学骨关节炎分级评估关节软骨的完整性。5个月运动后，运动对胶原网络的组织结构有影响，尤其是在中深层。然而，运动后没有检测到对机械性能的影响。在12个月大之前，跑步者比对照组表现出更少的骨关节炎，而在15个月大时，情况正好相反。它的结论是，在仓鼠，在年轻的时候，体育锻炼增强软骨成熟和改变的深度明智的软骨结构和组成。这可以被认为是有益的。然而，年轻时的锻炼对软骨有不良影响，骨关节炎的发病率显着增加。
Articular cartilage composition and structure are maintained and remodeled by chondrocytes under the influence of loading. Exercise-induced changes in the composition, structure, mechanical properties and tissue integrity of growing and aging hamster articular cartilage were investigated. Articular cartilage samples (n = 191) were harvested from the proximal tibiae of hamsters aged 1, 3, 6, 12 and 15 months. The hamsters were divided into runners and controls. The runners had free access to a running wheel between 1 and 3 months (runner groups 3-, 12- and 15-month-old hamsters) or 1 and 6 months (runner group 6-month-old hamsters) of age. Control animals were subjected to a sedentary lifestyle. Mechanical indentation tests and depth-wise compositional and structural analyses were performed for the cartilage samples. Furthermore, the integrity of articular cartilage was assessed using histological osteoarthritis grading. Exercise affected the collagen network organization after a 5-month exercise period, especially in the middle and deep zones. However, no effect on the mechanical properties was detected after exercise. Before the age of 12 months, the runners showed less osteoarthritis than the controls, whereas at 15 months of age the situation was reversed. It is concluded that, in hamsters, physical exercise at a young age enhances cartilage maturation and alters the depth-wise cartilage structure and composition. This may be considered beneficial. However, exercise at a young age demonstrated adverse effects on cartilage at a later age with a significant increase in the incidence of osteoarthritis.