PHENOTYPIC AND FUNCTIONAL-HETEROGENEITY OF HUMAN CLONED NATURAL-KILLER CELL-LINES
PHENOTYPIC AND FUNCTIONAL-HETEROGENEITY OF HUMAN CLONED NATURAL-KILLER CELL-LINES
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DOI：
10.1038/301158a0
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发表时间：
1983-01-01
期刊：
影响因子：
64.8
通讯作者：
RITZ, J
中科院分区：
文献类型：
--
作者：
HERCEND, T;REINHERZ, EL;RITZ, J
最近已经做出了广泛的努力来表征能够介导自然杀伤活性的细胞（参见参考文献1的综述），并且越来越多的证据表明这些细胞是异质的2，3。通过使用我们最近开发的用于克隆来自外周血的自然杀伤（NK）细胞的方法，我们分析了人类NK细胞的异质性。建立了7株具有NK活性的细胞系，并进行了4个月的研究。它们的表型用一系列单克隆抗体确定;抗T1、抗T3、抗T4、抗T8、抗T11、抗T12、抗Mo 1，并且每个细胞系似乎具有独特的表型。此外，尽管这些细胞系中的一些只能杀死K562细胞，NK活性的标准测定，但其他细胞系显示出对各种人类肿瘤和病毒转化细胞系的广泛但不同的反应谱。综上所述，这些结果证明了NK效应细胞的表型和功能异质性，最近在人类和海洋系统中均提出了这一点2，3，5。
Extensive efforts have recently been made to characterize cells capable of mediating natural killing activity (see ref. 1 for review) and increasing evidence has arisen that these cells were heterogeneous2,3. By using the methods we have recently developed for cloning natural killer (NK) cells derived from peripheral blood4, we have analysed the heterogeneity of human NK cells. Seven cell lines showing NK activity were established and studied for 4 months. Their phenotype was determined with a series of monoclonal antibodies; anti-T1, -T3, -T4, -T8, -T11, -T12, Mo1 and each cell line appeared to have a unique phenotype. Moreover, whereas some of these lines could only kill K562 cells, the standard assay of NK activity, others displayed a broad but distinct spectrum of reactivity against a variety of human tumour and viral transformed cell lines. Taken together, these results demonstrate the phenotypic and functional heterogeneity of NK effector cells which has recently been suggested in both human and marine systems2,3,5.