RKIP inhibits the malignant phenotypes of gastric cancer cells
RKIP inhibits the malignant phenotypes of gastric cancer cells
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RKIP抑制胃癌细胞的恶性表型
DOI：
10.4149/neo_2013_026
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发表时间：
2013-01-01
期刊：
影响因子：
3
通讯作者：
Zhang, G. Y.
中科院分区：
文献类型：
--
作者：
Zhang, X. M.;Gu, H.;Zhang, G. Y.
Raf激酶抑制蛋白（RKIP）是Raf-1的一种相互作用蛋白。RKIP表达在源自转移性乳腺癌、前列腺癌和黑色素瘤细胞的几种已建立的细胞系中是低的或不存在。然而，RKIP在胃癌中的功能作用仍不清楚。本研究以人胃癌细胞系SGC 7901为模型，对RKIP在胃癌细胞中的表达进行了重组。生长曲线和软琼脂实验显示RKIP对胃癌细胞株SGC 7901的生长和克隆形成有抑制作用。流式细胞仪分析显示RKIP可抑制胃癌细胞株SGC 7901的细胞周期进程并诱导其凋亡。创伤愈合和transwell侵袭实验显示RKIP抑制了SGC 7901细胞的迁移和侵袭。此外，我们观察到RKIP抑制SMGC 7901细胞在裸鼠移植瘤中的生长。综上所述，我们的体外和体内数据表明，RKIP调节胃癌7901细胞的增殖，凋亡，迁移，侵袭和致瘤性。这些结果揭示了RKIP在胃癌中的抑癌作用，提示RKIP可能成为胃癌治疗的新靶点。
Raf kinase inhibitor protein (RKIP) is first identified as an interacting partner of Raf-1. RKIP expression is low or absent in several established cell lines derived from metastatic breast cancer, prostate cancer and melanoma cells. However, the functional role of RKIP in gastric cancer remains unclear. In this study, we employed human gastric cancer cell line SGC7901 as a model to reconstitute RKIP expression in gastric cancer cells. The growth curve and soft agar assay showed that RKIP inhibited the growth and clonogenicity of SGC7901 cells. Flow cytometry analysis showed that RKIP inhibited the cell cycle progression and induced the apoptosis of SGC7901 cells. Wound healing and transwell invasion assay showed that RKIP inhibited the migration and invasion of SGC7901 cells. Furthermore, we observed that RKIP inhibited the growth of SMGC7901 cells in xenografts in nude mice. Taken together, our in vitro and in vivo data demonstrate that RKIP modulates the proliferation, apoptosis, migration, invasion and tumorigenicity of SGC7901 cells. These results reveal the tumor suppressor role of RKIP in gastric cancer and suggest that RKIP may be new therapeutic target for gastric cancer.