Monitoring of beet armyworm Spodoptera exigua (Lepidoptera: Noctuidae) resistance to chlorantraniliprole in China
Monitoring of beet armyworm Spodoptera exigua (Lepidoptera: Noctuidae) resistance to chlorantraniliprole in China
复制标题
中国甜菜夜蛾（鳞翅目：夜蛾科）对氯虫苯甲酰胺的抗药性监测
DOI：
10.1016/j.pestbp.2011.09.006
复制
发表时间：
2011-11-01
影响因子：
4.7
通讯作者：
Su, Jianya
中科院分区：
文献类型：
--
作者：
Lai, Tiancai;Li, Jia;Su, Jianya
甜菜夜蛾（Spodoptera exigua）是我国蔬菜上的主要害虫，对多种广谱杀虫剂产生了抗药性。最近注册的氯虫苯甲酰胺为控制这种对其他常规杀虫剂具有抗性的害虫提供了一种新的选择。用新生儿饲料掺入法测定了18个野外种群的敏感性，结果表明，18个野外种群的敏感性差异较大，LC（50）值在0.039 ~ 0.240mg/L之间。18个田间种群中有8个种群对氯虫苯甲酰胺表现出中等抗性水平，其余8个种群对氯虫苯甲酰胺表现出低水平抗性，仅1个种群对氯虫苯甲酰胺表现敏感。进行生化测定以确定参与耐受性变化的潜在机制。田间种群表现出不同的解毒酶活性，但氯虫苯甲酰胺毒性和酶活性之间的回归分析表明，每个田间种群可能有特定的生化机制的耐受性。用氯虫苯甲酰胺进行室内人工选择，连续选择23代，抗性提高11.8倍，多功能氧化酶和酯酶活性分别提高3.0倍和3.7倍。与室内敏感品系相比，筛选品系对该杀虫剂产生了128.6倍的抗性。增效试验表明，解毒酶可能不参与田间种群和选育菌株的抗性。(C)2011 Elsevier Inc. All rights reserved.
Beet armyworm, Spodoptera exigua is a major insect pest of vegetables in China, and has been reported to develop resistance to many broad-spectrum insecticides. Recently registered chlorantraniliprole provides a novel option for control of this pest resistant to other conventional insecticides. The susceptibilities of field collected populations were measured by diet incorporation assay with neonate, obvious variation of susceptibility was observed among the 18 field populations with LC(50) values varying from 0.039 to 0.240 mg/liter. Moderate resistant level was discovered in 8 of 18 field populations, other 8 populations had become low level tolerance to chlorantraniliprole, and only one population in all the field colonies remained susceptible. Biochemical assays were performed to determine the potential mechanisms involved in tolerance variation. Field populations displayed varied detoxification enzyme activities, but the regression analysis between chlorantraniliprole toxicities and enzyme activities demonstrated each field population might have specific biochemical mechanisms for tolerance. Artificial selection in laboratory with chlorantraniliprole was carried out, 23 generations of continuous selections resulted in 11.8-fold increase in resistance to chlorantraniliprole, and 3.0-fold and 3.7-fold increases in mixed function oxidase and esterase, respectively. Compared with the susceptible strain kept in laboratory the selection strain had developed 128.6-fold resistance to this insecticide. Synergism assays showed the detoxification enzymes might not involved in the resistance observed in field collected populations and the selected strain. (C) 2011 Elsevier Inc. All rights reserved.