Confocal imaging reveals three-dimensional fine structure difference between ventral and dorsal nerve roots.
Confocal imaging reveals three-dimensional fine structure difference between ventral and dorsal nerve roots.
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DOI：
10.1117/1.3575167
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发表时间：
2011-05
影响因子：
3.5
通讯作者：
Yuxiang Wu;T. Sui;Xiao-Jian Cao;Xiaohua Lv;Shaoqun Zeng;P. Sun
中科院分区：
文献类型：
--
作者：
Yuxiang Wu;T. Sui;Xiao-Jian Cao;Xiaohua Lv;Shaoqun Zeng;P. Sun
周围神经损伤修复是神经外科中最具挑战性的问题之一，部分原因是对周围神经的三维精细结构和组织缺乏了解。本文用激光扫描共聚焦显微镜观察了腹神经和背神经的神经纤维结构。厚组织染色结果表明，腹神经和背神经的神经纤维具有不同的三维结构，连续切片图像重建显示，腹神经的神经纤维以卷曲的形式运动，而在背神经的神经纤维以平行的索状形式运动。这些结构差异有助于外科医生在周围神经损伤修复中区分腹神经和背神经，也有助于科学家对神经纤维组织有更深入的了解。
Peripheral nerve injury repair is one of the most challenging problems in neurosurgery, partially due to lack of knowledge of three-dimensional (3-D) fine structure and organization of peripheral nerves. In this paper, we explored the structures of nerve fibers in ventral and dorsal nerves with a laser scanning confocal microscopy. Thick tissue staining results suggested that nerve fibers have a different 3-D structure in ventral and dorsal nerves, and reconstruction from serial sectioning images showed that in ventral nerves the nerve fibers travel in a winding form, while in dorsal nerves, the nerve fibers form in a parallel cable pattern. These structural differences could help surgeons to differentiate ventral and dorsal nerves in peripheral nerve injury repair, and also facilitate scientists to get a deeper understanding about nerve fiber organization.