Screening for previous refractive surgery in eye bank corneas by using optical coherence tomography
Screening for previous refractive surgery in eye bank corneas by using optical coherence tomography
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DOI：
10.1097/ico.0b013e31803c5535
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发表时间：
2007-06-01
期刊：
影响因子：
2.8
通讯作者：
Huang, David
中科院分区：
文献类型：
--
作者：
Lin, Roger C.;Li, Yan;Huang, David
目的：使用光学相干断层扫描 (OCT) 来检测供体角膜的既往屈光手术情况。方法：我们构建了一台工作波长为 1310 nm 的桌面 OCT 扫描仪。克利夫兰眼库的供体角膜被密封在浸泡在 Optisol GS 中的无菌容器内进行扫描。使用 7.6 毫米长的线沿 8 条经线进行 OCT 扫描（512 次轴向扫描）。使用我们开发的图像处理软件自动绘制角膜前表面和后角膜表面。曲率是根据中心 5 毫米直径的最佳抛物线拟合计算得出的。还进行了基质反射率的分层分析。对来自 19 名捐献者的 29 个角膜进行了检查。 5 例有激光原位角膜磨镶术 (LASIK) 病史。结果：瓣界面无法在裂隙灯或 OCT 图像上可视化，但通过组织学证实。正常角膜的死亡至扫描时间为 22.1 +/- 11.4 (SD) 小时，LASIK 角膜为 100.6 +/- 57.5 小时。对照角膜的前表面屈光度为 67.5 +/- 2.5 D，LASIK 角膜的前表面屈光度为 64.5 +/- 2.4 D (P = 0.023)。两组后曲率和厚度参数之间没有显着性差异。 LASIK 术后角膜中央 4 毫米直径的前/后反射率比显着低于对照组（P < 0.05）。结论：OCT 提供供体角膜的厚度、地形和反射率图，而无需将其从保存介质和容器中取出。前曲率和前/后基质反射率比可用于检测先前的 LASIK。
Purpose: To use optical coherence tomography (OCT) to detect previous refractive surgery in donor corneas.Methods: We constructed a tabletop OCT scanner operating at 1310-nm wavelength. Donor corneas at the Cleveland Eye Bank were scanned while sealed within the sterile container immersed in Optisol GS. OCT scanning was performed with 7.6-mm-long lines (512 axial scans) along 8 meridians. Anterior and posterior corneal surfaces were automatically mapped using image processing software that we developed. Curvature was computed from the best parabolic fit in the central 5-mm diameter. Layered analysis of the stromal reflectivity was also performed. Twenty-nine corneas from 19 donors were examined. Five had a history of laser in situ keratomileusis (LASIK).Results: The flap interfaces could not be visualized on slit-lamp or OCT images but were confirmed by histology. The death-to-scan time was 22.1 +/- 11.4 (SD) hours for normal corneas and 100.6 +/- 57.5 hours for LASIK corneas. The anterior surface power was 67.5 +/- 2.5 D in control corneas and 64.5 +/- 2.4 D in LASIK corneas (P = 0.023). There was no significance between the 2 groups in terms of posterior curvature and thickness parameters. The anterior/posterior reflectivity ratio in the central 4-mm diameter was significantly lower in post-LASIK corneas than in control (P < 0.05).Conclusions: OCT provides thickness, topography, and reflectivity maps of donor corneas without taking them out of preservation medium and container. The anterior curvature and the anterior/posterior stromal reflectivity ratio may be useful for detecting previous LASIK.