Pattern formation in the cerebellum of murine embryonic stem cell chimeras
Pattern formation in the cerebellum of murine embryonic stem cell chimeras
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DOI：
10.1046/j.1460-9568.1998.00085.x
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发表时间：
1998-02-01
影响因子：
3.4
通讯作者：
Tan, SS
中科院分区：
文献类型：
--
作者：
Hawkes, R;Faulkner-Jones, B;Tan, SS
小脑皮质被细分为一系列精细的、定型的横带和旁带。据推测，（i）所有的浦肯野细胞来源于胚胎发生早期分配的10至20种前体细胞，（ii）模式形成是基于创始人库中的细胞谱系限制。这些假设已经通过胚胎干细胞嵌合体的克隆分析进行了验证。这两种推测都不成立：分析表明，浦肯野细胞来源于一个> 10（2）个前体细胞的建立者群体，小脑横向发育边界和小脑旁条纹都不是克隆起源的。我们的结论是早期谱系限制在小脑模式形成中没有作用。
The cerebellar cortex is subdivided into an elaborate, stereotyped array of transverse zones and parasagittal stripes. It has been speculated that (i) all Purkinje cells derive from 10 to 20 precursors allocated early in embryogenesis and (ii) that pattern formation is based on cell lineage restriction in the founder pool. These hypotheses have been tested by clonal analysis of embryonic stem cell chimeras. Neither speculation is supported: the analysis suggests that Purkinje cells derive from a founder population of > 10(2) precursors, and that neither cerebellar transverse developmental boundaries nor parasagittal stripes have a clonal origin. We conclude that early lineage restriction plays no role in cerebellar pattern formation.