Nucleotide conformational analysis by 31P nuclear magnetic resonance spectroscopy.
Nucleotide conformational analysis by 31P nuclear magnetic resonance spectroscopy.
复制标题
通过 31P 核磁共振波谱进行核苷酸构象分析。
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
D. Gorenstein
中科院分区：
文献类型：
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作者：
D. Gorenstein
随着傅里叶变换（F1）和高场核磁共振（NMR）谱仪的广泛应用，生物磷酸盐的31 P NMR谱现已变得相当普遍。这并不奇怪，因为31 p核具有便利的NMR特性：自旋1/2，100%天然丰度，适度的弛豫时间，广泛的化学位移，以及在许多生物分子结构中的关键作用。傅立叶变换NMR大大减少了在生物系统中使用31 p NMR的一个严重限制，即磷核的低灵敏度（与1H NMR相比，在恒定场为6.6%）。磷原子核的浓度可方便地监测到100、100、100毫摩尔（或更低）。NMR光谱信息包括共振线位置（化学位移，δ）、自旋-自旋耦合常数（J）、自旋-晶格（TI）和自旋-自旋（1 i）弛豫时间。此外，信号区域通常与核浓度直接相关。有了这些参数，核磁共振光谱提供了一个独特的，非微扰探针在解决方案的结构和时间依赖性的分子性质。这篇评论的大部分内容与31 p化学位移有关。有效地使用31 p NMR作为探针的核苷酸构象需要了解的结构和环境因素，影响这些变化。因此，我将首先讨论一些改变
With the widespread utilization of Fourier transform (F1) and high field nuclear magnetic resonance (NMR) spectrometers, 31 P NMR spectroscopy of biological phosphates has now become quite common. This is not surprising, since the 31p nucleus has convenient NMR properties: spin 1/2, 100% natural abundance, moderate relaxation times, wide range of chemical shifts, and a key role in many biomolecu lar structures. Fourier transform NMR has substantially reduced the one serious limitation to the use of 31p NMR in biological systems, which is the low sensitivity of the phosphorus nucleus (6.6% at constant field compared to IH NMR). Routinely, millimolar (or lower) con centrations of phosphorus nuclei are conveniently monitored. NMR spectroscopic information includes the resonant line positions (chemical shifts, 8), the spin-spin coupling constants (J), and spin-lattice (TI) and spin-spin (1;) relaxation times. In addition, signal areas are often directly related to nuclei concentration. With these parameters NMR spectroscopy provides a unique, nonperturbing probe in solution of the structure and time-dependent properties of molecules. Much of this review is concerned with 31p chemical shifts. Effective use of 31p NMR as a probe of nucleotide conformation requires an understanding of the structural and environmental factors that influence these shifts. I will therefore first discuss some of the factors that alter