ON ROLE OF SOLUBLE RIBONUCLEIC ACID IN CODING FOR AMINO ACIDS
ON ROLE OF SOLUBLE RIBONUCLEIC ACID IN CODING FOR AMINO ACIDS
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DOI：
10.1073/pnas.48.6.1086
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发表时间：
1962-01-01
影响因子：
11.1
通讯作者：
LIPMANN, F
中科院分区：
文献类型：
--
作者：
CHAPEVILLE, F;EHRENSTEIN, GV;LIPMANN, F
一个直接测试的假设，sRNA的功能作为一个适配器，在指定的模板上的氨基酸的配合。半胱氨酸，连接到其正常受体sRNA的转化，而仍然连接到丙氨酸，通过还原与阮内镍。所得杂交体的编码特性通过使用聚UG来检查，所述聚UG刺激半胱氨酸而不是丙氨酸掺入肽中。与半胱氨酸受体连接的丙氨酸被掺入，表明衔接子假说是正确的。
A direct test is made of the hypothesis that sRNA functions as an adaptor in specifying the fit of amino acids on a template. Cystein, attached to its normal acceptor sRNA is converted, while still attached to alanine, by reduction with Raney Nickel. The coding properties of the resultant hybrid are examined by the use of poly UG which stimulates incorporation into peptide of cysteine but not alanine. Alanine, attached to the cystein acceptor is incorporated, indicating that the adaptor hypothesis is correct.