Homodirectional changes in transcriptome composition and mRNA translation induced by rapamycin and heat shock
Homodirectional changes in transcriptome composition and mRNA translation induced by rapamycin and heat shock
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DOI：
10.1038/nsb1015
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发表时间：
2003-12-01
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影响因子：
--
通讯作者：
Hentze, MW
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作者：
Preiss, T;Baron-Benhamou, J;Hentze, MW
转录和 mRNA 更新决定细胞转录组的数量组成。转录组又通过翻译充当蛋白质组的模板。用 TOR 激酶抑制剂雷帕霉素处理酿酒酵母会导致数百个基因的 mRNA 水平增加或减少。我们使用 DNA 微阵列分析来同时监测所有可检测酵母 mRNA 的转录组和翻译变化。值得注意的是，转录组中诱导的基因与更有效翻译的 mRNA 紧密相关（基于它们与多核糖体关联的相对程度）；同样，雷帕霉素处理后 mRNA 水平降低的基因也表现出较低的翻译适应性。对热休克细胞的微阵列分析也揭示了同向共同调节反应。因此，信号诱导的转录组变化在翻译水平上被放大。这些结果揭示了更高水平的协调基因调控，我们称之为增强。
Transcription and mRNA turnover determine the quantitative composition of the cellular transcriptome. The transcriptome in turn serves as a template for the proteome via translation. Treatment of Saccharomyces cerevisiae with the TOR kinase inhibitor rapamycin causes increases and decreases in the mRNA levels of hundreds of genes. We used DNA microarray analysis to monitor simultaneously transcriptome and translational changes for all detectable yeast mRNAs. Notably, genes that are induced in the transcriptome correlate tightly with more efficiently translated mRNAs ( based on their relative degree of polyribosome association); similarly, genes that show reduced mRNA levels after rapamycin treatment also show lower translational fitness. Microarray analyses on heat-shocked cells also reveal homodirectional co-regulatory responses. Thus, signal-induced changes in the transcriptome are amplified at the translational level. These results unveil a higher level of coordinated gene regulation that we refer to as potentiation.