Novel fluorescent protein from Discosoma coral and its mutants possesses a unique far-red fluorescence
Novel fluorescent protein from Discosoma coral and its mutants possesses a unique far-red fluorescence
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DOI：
10.1016/s0014-5793(00)01895-0
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发表时间：
2000-08-18
期刊：
影响因子：
3.5
通讯作者：
Lukyanov, SA
中科院分区：
文献类型：
--
作者：
Fradkov, AF;Chen, Y;Lukyanov, SA
从盘体珊瑚中克隆了一个新的高级红移蛋白基因，其最大发射波长为593 nm。该蛋白命名为dsFP 593，与最近描述的GFP样蛋白drFP 583高度同源，其最大发射波长为583 nm。利用drFP 583和dsFP 593基因的显著相似性，我们进行了“改组”程序以产生由两个基因的不同部分组合组成的突变体库。选择一个“杂合基因”用于随后的随机诱变，其导致在616 nm处具有独特红移发射最大值的突变变体，（C）2000欧洲生物化学学会联合会。由Elsevier Science B.V.出版，版权所有。
A novel gene for advanced red-shifted protein with an emission maximum at 593 nm was cloned from Discosoma coral. The protein, named dsFP593, is highly homologous to the recently described GFP-like protein drFP583 with an emission maximum at 583 nm, Using the remarkable similarity of the drFP583 and dsFP593 genes, we performed a 'shuffling' procedure to generate a pool of mutants consisting of various combinations of parts of both genes. One 'hybrid gene' was chosen for subsequent random mutagenesis, which resulted in a mutant variant with a uniquely red-shifted emission maximum at 616 nm, (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.