Immunohistochemical Analysis of the Proteolytic Enzyme Subtilase in the Digestive Organs of the Sea Urchin Strongylocentrotus intermedius
Immunohistochemical Analysis of the Proteolytic Enzyme Subtilase in the Digestive Organs of the Sea Urchin Strongylocentrotus intermedius
复制标题
海胆中间圆甲藻消化器官中蛋白水解酶枯草杆菌酶的免疫组织化学分析
DOI：
10.1002/ar.21484
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Y.Takagi
中科院分区：
文献类型：
--
作者：
K.Ura;S.Hasegawa;E.Tanaka;T.Gotoh;T.Ojima;Y.Takagi
枯草杆菌酶是短刺海胆（中间球海胆）中的一种主要蛋白酶，分离并用作随后生产特异性多克隆抗体的抗原。免疫组化分析显示，在前胃、后胃和前肠的颗粒中有免疫反应细胞。这些颗粒在前胃中数量最多，也被亚甲蓝-天青II强烈染色。然而，食管，后肠，直肠细胞中的颗粒没有被这种抗体染色。我们的结论是，枯草杆菌酶主要定位于胃和前肠的海胆。Anat Rec，2012.© 2011 Wiley Periodicals，Inc.
Subtilase, a major protease in the short‐spined sea urchin (Strongylocentrotus intermedius), was isolated and used as antigen for the subsequent production of a specific polyclonal antibody. Immunoreactive cells were observed by immunohistochemical analysis in granules in the anterior and posterior stomach and the anterior intestine. These granules, which were most numerous in the anterior stomach, also stained intensely with methylene blue‐Azure II. However, granules in cells of the esophagus, posterior intestine, and rectum were not stained by this antibody. We conclude that subtilase mainly localizes in the stomach and anterior intestine of the sea urchin. Anat Rec, 2012. © 2011 Wiley Periodicals, Inc.