In Vivo Confocal Imaging of Fluorescently Labeled Microbubbles: Implications for Ultrasound Localization Microscopy
In Vivo Confocal Imaging of Fluorescently Labeled Microbubbles: Implications for Ultrasound Localization Microscopy
复制标题
DOI：
10.1109/tuffc.2020.2988159
复制
发表时间：
2020-09-01
影响因子：
3.6
通讯作者：
Song, Pengfei
中科院分区：
文献类型：
--
作者：
Lowerison, Matthew R.;Huang, Chengwu;Song, Pengfei
我们报告了通过共焦显微镜测量的毛细血管水平微脉管系统中荧光标记的微泡（MB）的时间动力学，并将这些结果与超声定位显微镜（ULM）进行比较。观察到的每个共焦视场 (212 μ m x 212 μ m) 的 19.4 +/- 4.2 MB 与每帧 19.1 MB 的预期计数非常一致。完全灌注该毛细血管网络的估计时间为 193 秒，这证实了文献中报告的值。然后，我们将毛细管网络建模为根据经验确定的离散时间马尔可夫链，通过各个毛细管具有可调节的 MB 转移概率。蒙特卡罗随机游走模拟发现灌注时间范围从无偏马尔可夫链的 24.5 秒到异质流分布的 182 秒。这项试点研究证实了对超分辨率 ULM 所需的长采集时间的概率推导解释。
We report the time kinetics of fluorescently labeled microbubbles (MBs) in capillary-level microvasculature as measured via confocal microscopy and compare these results to ultrasound localization microscopy (ULM). The observed 19.4 +/- 4.2 MBs per confocal field-of-view (212 mu m x 212 mu m) are in excellent agreement with the expected count of 19.1 MBs per frame. The estimated time to fully perfuse this capillary network was 193 s, which corroborates the values reported in the literature. We then modeled the capillary network as an empirically determined discrete-time Markov chain with adjustable MB transition probabilities though individual capillaries. The Monte Carlo random walk simulations found perfusion times ranging from 24.5 s for unbiased Markov chains up to 182 s for heterogeneous flow distributions. This pilot study confirms a probability-derived explanation for the long acquisition times required for super-resolution ULM.