Two promoters direct expression of the murine Spi-B gene, an Ets family transcription factor
Two promoters direct expression of the murine Spi-B gene, an Ets family transcription factor
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DOI：
10.1016/s0378-1119(97)00629-x
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发表时间：
1998-01-30
期刊：
影响因子：
3.5
通讯作者：
Tenen, DG
中科院分区：
文献类型：
--
作者：
Chen, HM;Gonzalez, DA;Tenen, DG
Spi-B和PU.1（Spi-I）构成Ets转录因子家族中最分歧的亚家族。Spi-B和PU.1结合到相似的DNA序列，并且可以在体外激活相同的B细胞和骨髓启动子。然而，PU.1敲除小鼠表现出多种造血谱系的造血发育缺陷，表明Spi-B不能补偿PU.1的损失。对这些结果的一种解释是，与在骨髓和B细胞系中表达的PU. 1相反，Spi-B表达仅限于B细胞。为了开始理解Spi-B基因的调控，分离鼠Spi-B cDNA和基因组克隆。外显子/内含子组织和转录起始位点进行了测定，两个主要的转录起始位点进行了检测。两个Spi-B启动子进行了分离和表征，并在B细胞系中显示出与内源基因相匹配的差异活性。对这两个Spi-B启动子的进一步研究将为深入了解Spi-B在B细胞中的组织特异性和发育阶段特异性表达的分子事件提供帮助。(C)1998年Elsevier Science B.V.
Spi-B and PU.1 (Spi-1) comprise the most divergent subfamily of the Ets transcription factor family. Spi-B and PU.1 bind to similar DNA sequences, and can activate the same B-cell and myeloid promoters in vitro. However, PU.1 knockout mice demonstrate defective hematopoietic development of multiple hematopoietic lineages, indicating that Spi-B was not able to compensate for loss of PU.1. One explanation for these results is that, in contrast to PU.1, which is expressed in myeloid and B-cell lines, Spi-B expression is restricted to B-cells. In order to begin to understand the control of regulation of the Spi-B gene, murine Spi-B cDNA and genomic clones were isolated. The exon/intron organization and transcriptional start sites were determined; two major transcriptional start sites were detected. The two Spi-B promoters were isolated and characterized, and displayed differential activity in B-cell lines matching that of the endogenous gene. Further study of the two Spi-B promoters will provide insight into the molecular events regulating the tissue-specific and developmental stage-specific expression of Spi-B in B-cells. (C) 1998 Elsevier Science B.V.