Effects of ethanol on monoamine and amino acid release from cerebral cortical slices of the alcohol-preferring P line of rats.
Effects of ethanol on monoamine and amino acid release from cerebral cortical slices of the alcohol-preferring P line of rats.
复制标题
乙醇对嗜酒 P 系大鼠大脑皮层切片单胺和氨基酸释放的影响。
DOI：
10.1111/j.1530-0277.1986.tb05072.x
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Li,TK
中科院分区：
文献类型：
--
作者：
McBride,WJ;Murphy,JM;Lumeng,L;Li,TK
本文研究了250 mg/100ml乙醇对3，4-二羟基苯乙酸(DOPAC)外流和35nW K+刺激的去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)、γ-氨基丁酸、谷氨酸和天冬氨酸(Asp)的影响。在K+刺激下，P组大鼠依赖于钙离子的GABA释放量降低了35%，而谷氨酸释放量几乎是对照组的两倍。两组其他化合物的释放情况相似。在灌流液中加入250 mg/100ml乙醇，不影响两组大鼠脑片NE、DA和5-羟色胺的释放，也不影响DOPAC的外流。然而，无论是P组还是STOCK组的大鼠，乙醇对K+刺激的、依赖于钙离子的GABA、谷氨酸和天冬氨酸的释放都有显著的促进作用。这些体外实验数据不支持乙醇对多巴胺、去甲肾上腺素和5-羟色胺的释放或DOPAC外流的直接作用，但表明乙醇对大脑皮层GABA、谷氨酸和天冬氨酸的递质释放过程有直接作用。
The effects of 250 mg/100 ml ethanol on the efflux of 3,4‐dihydrox‐yphenylacetic acid (DOPAC) and the 35 nw K+‐stimulated, Ca2+‐dependent release of norepinephrine (NE), dopamine (DA), serotonin (5‐HT), γ‐aminobutyric acid (GABA), glutamate, and aspartate from cerebral cortical slices of the alcohol‐preferring P line of rats and stock Wistar rats were studied. The K+‐stimuiated, Ca2+‐dependent release of GABA for the P rats was 35% lower, while the release of glutamate was almost twice that of the stock animals. The release of the other compounds was similar for the two groups. Addition of 250 mg/100 ml ethanol to the superfusion media did not after the release of NE, DA, and 5‐HT nor the efflux of DOPAC from cortical slices of either group of rats. However, the K+‐stimulated, Ca2+‐dependent release of GABA, glutamate, and aspartate was significantly enhanced by ethanol for both the P and stock group of rats. These in vitro data do not support a direct action of ethanol on DA, NE, and 5‐HT release or on DOPAC efflux, but suggest a direct action on the transmitter release process for GABA, glutamate, and aspartate in the cerebral cortex.