Biostimulation of dermal fibroblast by sublethal Q-switched Nd:YAG 532 nm laser: collagen remodeling and pigmentation.
Biostimulation of dermal fibroblast by sublethal Q-switched Nd:YAG 532 nm laser: collagen remodeling and pigmentation.
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DOI：
10.1016/j.jphotobiol.2005.05.006
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发表时间：
2005-10
期刊：
影响因子：
--
通讯作者：
V. Poon;Lin Huang;A. Burd
中科院分区：
文献类型：
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作者：
V. Poon;Lin Huang;A. Burd
医用激光在治疗色素性病变中的应用在过去十年中迅速发展。在临床和美容应用中，黑色素被靶向色素区域并通过选择性光热解机制被破坏。当激光辐射穿过浅层色素组织时，能量会因真皮胶原蛋白的散射和吸收而进一步减少。非色素真皮成纤维细胞将同时受到较低能量水平的激光照射。本文报道了低能量激光对真皮成纤维细胞的生物刺激。本研究采用临床激光治疗中使用的调Q倍频Nd:YAG 532nm激光器。亚致死激光能量密度测定为 0.8J/cm2，用于刺激单层培养中的正常人成纤维细胞。结果显示，受刺激的成纤维细胞和对照组之间的胶原蛋白合成没有显着差异。然而，通过测量成纤维细胞填充的胶原蛋白晶格（FPCL）收缩，表明照射组中胶原蛋白重塑活性显着延迟。通过 RT-PCR 分析确定了 SCF、HGF 和 b-FGF 基因表达的刺激情况，并证明该刺激因病例而异。六分之二的细胞谱系对激光刺激对 SCF、HGF 和 b-FGF 基因表达表现出更强的反应，用于制备条件培养基。照射组的条件培养基显示SCF和b-FGF含量显着增加，并刺激SK-mel-3黑色素瘤细胞在体外合成更多黑色素。这些结果表明，成纤维细胞的亚致死激光刺激可能通过产生黑色素刺激性细胞因子而导致激光后色素沉着过度。各个细胞谱系之间 SCF、HGF 和 b-FGF 产生的刺激程度有所不同，这可能反映了临床实践中激光后色素沉着过度的真实变化。
The application of medical lasers in treating pigmented lesions has rapidly developed over the past decade. In both clinical and cosmetic application, melanin is targeted in pigmented areas and destroyed by the mechanism of selective photothermolysis. When laser radiation passes through superficial pigmented tissue, energy will be further reduced by dermal collagen scattering and absorption. Non-pigmented dermal fibroblasts will be exposed to co-incidental laser irradiation at lower energy levels. Biostimulation of dermal fibroblasts by low energy laser is reported in this paper. The Q-switched frequency doubled Nd:YAG 532nm laser used in clinical laser therapy was used in this study. Sublethal laser fluence was determined at 0.8J/cm2and used to stimulate normal human fibroblasts in monolayer culture. The results showed that there was no significant difference in collagen synthesis between the stimulated fibroblasts and controls. However, significant delay in collagen remodeling activity was demonstrated in the irradiated group by measuring fibroblast populated collagen lattice (FPCL) contraction. The stimulation of SCF, HGF and b-FGF gene expression was determined by RT-PCR analysis and demonstrated to vary between cases. Two out of six cell lineages that showed stronger responses to laser stimulation on SCF, HGF and b-FGF gene expressions were used to prepare conditioned media. The conditioned media from irradiated groups showed significant increase in SCF and b-FGF content and stimulated SK-mel-3 melanoma cells to synthesize more melanin in vitro. These results suggest that sublethal laser stimulation of fibroblasts may cause post-laser hyperpigmentation through production of melanogenic stimulatory cytokines. The degree of stimulation of SCF, HGF and b-FGF production varied between individual cell lineages, which may reflect the true variation of post-laser hyperpigmentation in clinical practice.