Sequence and phylogenetic analyses of the chloroplast 16S rRNA, tufA, and rbcL genes from Bryopsis hypnoides
Sequence and phylogenetic analyses of the chloroplast 16S rRNA, tufA, and rbcL genes from Bryopsis hypnoides
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羽藻叶绿体 16S rRNA、tufA 和 rbcL 基因的序列和系统发育分析
DOI：
10.1007/s00343-011-0084-9
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发表时间：
2011-08
影响因子：
--
通讯作者：
Wang Guangce
中科院分区：
文献类型：
--
作者：
Lue Fang;Lue Fang;Wang Guangce;Wang Guangce
利用鸟枪法测序技术，获得了中国青岛产催眠真藓（Bryopsis hypnoides）叶绿体16S rRNA和tufA基因的全序列。16SrRNA基因中有两个I组内含子，其结构类似于蕨藻（Caulerpasertularioides）（苔藓植物目，绿藻门）。叶绿体编码的tufA基因序列长1230bp，富含AT（61.5%），与已发表的bryopsidinea藻类16SrRNA序列相似。基于叶绿体16S rRNA和tufA基因序列数据的系统发育分析支持以前的假设，Bryopsidineae，Halimedineae和Ostreobidineae是三个不同的谱系。这些结果也证实了Avrainvillea从Udoteaceae中排除。系统发育分析表明，该属是Derbesia的姐妹属，但该分支缺乏强大的节点支持。此外，从rbcL基因库序列推断的系统发育树，结合苔藓植物的地理分布，确定了一个强有力的支持支三个不同分布的亚洲苔藓植物种。初步结果表明，这些生物具有明显的区域特有性。
Using shotgun sequencing data, the complete sequences of chloroplast 16S rRNA and tufA genes were acquired from native specimens of Bryopsis hypnoides (Qingdao, China). There are two group I introns in the 16S rRNA gene, which is structurally similar to that of Caulerpa sertularioides (Bryopsidales, Chlorophyta). The chloroplast-encoded tufA gene sequence is 1 230 bp long, very AT-rich (61.5%), and is similar to previously published 16S rRNA sequences of bryopsidinean algae. Phylogenetic analyses based on chloroplast 16S rRNA and tufA gene sequence data support previous hypotheses that the Bryopsidineae, Halimedineae, and Ostreobidineae are three distinct lineages. These results also confirmed the exclusion of Avrainvillea from the family Udoteaceae. Phylogenetic analyses inferred that the genus Bryopsis as sister to Derbesia; however, this clade lacked robust nodal support. Moreover, the phylogenetic tree inferred from rbcL GenBank sequences, combined with the geographical distributions of Bryopsis species, identified a strongly supportive clade for three differently distributed Asian Bryopsis species. The preliminary results suggesting that these organisms are of distinct regional endemism.
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