Molecular cloning, characterization, mRNA expression changes and nucleocytoplasmic shuttling during kidney embryonic development of SOX9 in Alligator sinensis
Molecular cloning, characterization, mRNA expression changes and nucleocytoplasmic shuttling during kidney embryonic development of SOX9 in Alligator sinensis
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扬子鳄肾胚胎发育过程中SOX9的分子克隆、表征、mRNA表达变化和核质穿梭
DOI：
10.1016/j.gene.2020.144334
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发表时间：
2020
期刊：
影响因子：
3.5
通讯作者：
Tiechen Li
中科院分区：
文献类型：
--
作者：
Lin Wang;Ruiqing Cai;Fengnan Liu;Yang Lv;Ying Zhang;Shulong Duan;Ali Izaz;Jue Zhou;Hui Wang;Renjie Duan;Xiaobing Wu;Tiechen Li
SOX9通过在所有脊椎动物中通过SOM介导的正向自我调节，在体细胞组织发育和成体再生过程中发挥着重要的、广泛的和保守的作用。在本研究中，我们克隆了中华扬子鳄肾脏中的SOX9。该基因全长3878 ，开放阅读框编码494个氨基酸。氨基酸比对分析表明，SOX9具有高度保守的功能结构域。应用液滴数字聚合酶链式反应检测肾组织中SOX9基因的表达水平。中华绒毛虫在胚胎发育过程中表现出明显的变化，提示SOX9可能在复杂肾脏发育的调节中发挥重要作用。有趣的是，我们用免疫荧光检测到了SOX9蛋白的核质穿梭，这意味着核质穿梭在肾脏胚胎发育中对SOX9的调控至关重要。总之，这些数据为进一步研究肾脏发育生物学和非哺乳动物SOX9的分子生物学奠定了重要基础。此外，本研究还为扬子鳄SOX9核质穿梭现象提供了新的认识，这可能对肾后肾胚胎的发育具有重要意义。
SOX9 plays a crucial, extensive and conservative role in the process of somatic tissue development and adult regeneration through the positive self-regulation mediated by SOM across all vertebrates. In this study, we have cloned SOX9 from the kidney of hatchlingAlligator sinensis. The full-length of SOX9 cDNA is 3878 bp with an open reading frame encoding 494 amino acids. Amino acid alignment analyses indicated that the SOX9 exhibit highly conserved functional domains. Using the droplet digital PCR, the mRNA abundances of SOX9 during nephrogenesis inA. sinensisshowed prominent changes in the embryonic development, suggesting that SOX9 might combines a vital role in the regulation of complex renal development. Interestingly, we detected the nucleocytoplasmic shuttling of SOX9 protein using immunofluorescence, implying that nucleocytoplasmic shuttling is critical to the regulation of SOX9 in the renal embryonic development. Collectively, these data provide an important foundation for further studies on renal developmental biology and molecular biology of non-mammalian SOX9. Furthermore, it provides new insights into the phenomenon of SOX9 nucleocytoplasmic shuttling inAlligator sinensis, which is probably of great significance to the development of kidney metanephros embryo.