Ovarian steroids regulate 24p3 expression in mouse uterus during the natural estrous cycle and the preimplantation period
Ovarian steroids regulate 24p3 expression in mouse uterus during the natural estrous cycle and the preimplantation period
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DOI：
10.1677/joe.0.1620011
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发表时间：
1999-07-01
影响因子：
4
通讯作者：
Chen, YH
中科院分区：
文献类型：
--
作者：
Huang, HL;Chu, ST;Chen, YH
我们研究了24 p3在小鼠子宫中的表达在自然发情周期的各个阶段，并在植入前期。24 p3 mRNA在动情前期和动情期出现高峰，动情后期至动情间期急剧下降。与此相一致，24 p3蛋白在动情前期大量表达，动情后期逐渐下降，动情间期降至极低水平。在发情前期和发情期，子宫24 p3的表达与雌二醇（E-2）的高峰密切重叠，但在生殖周期中，当孕酮（P-4）升高到高水平时，子宫24 p3的表达受到抑制。在未成熟的小鼠或切除卵巢的成年动物的子宫中，既没有检测到蛋白质，也没有检测到其信息。虽然注射P-4的这些动物不能启动子宫24 p3的表达，雌激素类固醇给药这些动物显着刺激的基因表达。另一方面，用E-2和P-4一起处理这些动物，没有刺激基因表达。在妊娠动物中（第1天（D1）=阴道栓日），24 p3 mRNA在D1和D2保持在高水平，但在D3和D4下降到几乎检测不到的水平。24 p3蛋白在D1 ~ D2表达减少，D3 ~ D4表达减少至不可检测水平。24 p3蛋白免疫组化定位和24 p3 mRNA原位杂交检测结果表明，24 p3主要表达于子宫内膜的腔上皮细胞和腺上皮细胞。这与我们先前观察到的在子宫腔液中发现的24 p3蛋白一起表明，在发情前期和发情期，该蛋白主要从这些细胞分泌到它们各自的腔表面。
We examined 24p3 expression in the mouse uterus at various stages of the natural estrous cycle and during the preimplantation period. The level of 24p3 mRNA appeared intensively in proestrus and estrus, then declined sharply from metestrus to diestrus, Consistent with this observation, 24p3 protein was abundant in proestrus, decreased from estrus to metestrus and declined to a very low level in diestrus. The uterine 24p3 expression closely overlapped with the estradiol (E-2) surge in proestrus and estrus but it was suppressed when progesterone (P-4) rose to a high level during the reproductive cycle. Neither the protein nor its message was detected in the uteri of immature mice or ovariectomized adult animals. While an injection of P-4 to these animals was unable to initiate uterine 24p3 expression, administration of estrogenic steroids to these animals markedly stimulated the gene expression. Treatment of these animals with E-2 together with P-4 on the other hand, did not stimulate the gene expression. In pregnant animals (day 1 (D1)=day of vaginal plug), 24p3 mRNA remained at a high level on D1 and D2 but dropped to an almost undetectable level on D3 and D4. This was accompanied by a decrease in 24p3 protein from D1 to D2 and a decline in the protein to undetectable levels from D3 to D4, The staining patterns of both the immunohistochemical localization of 24p3 protein and in situ hybridization for the detection of 24p3 mRNA in the uterine sections showed that 24p3 expression took place mainly in the luminal and glandular epithelial cells of the endometrium. This together with our previous observation that 24p3 protein is found in uterine luminal fluid indicates that the protein is secreted primarily from these cells to their respective luminal surfaces during proestrus and estrus.