Fusion of Uniluminal Vascular Spheroids: A Model for Assembly of Blood Vessels
Fusion of Uniluminal Vascular Spheroids: A Model for Assembly of Blood Vessels
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DOI：
10.1002/dvdy.22161
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发表时间：
2010-02-01
影响因子：
2.5
通讯作者：
Drake, Christopher J.
中科院分区：
文献类型：
--
作者：
Fleming, Paul A.;Argraves, W. Scott;Drake, Christopher J.
我们评估了单层血管球体的自组装特性，该球体具有血管平滑肌细胞的外层和内衬中央管腔的内皮细胞的连续内层。我们发现，虽然对uniluminal血管球状体悬浮在培养基中融合，形成一个更大的直径的球状体结构，球状体在胶原蛋白水凝胶形成细长的结构。这些发现强调了单腔血管球体作为工程血管模块的潜在用途。我们还表明，uniluminal血管球体融合符合模型描述的液滴聚结。此外，在体外的uniluminal血管球体的融合非常类似于在体内的过程中，其中降主动脉形成的配对的背侧动脉在胚胎发育过程中的融合。总之，研究结果表明，组织流动性的基础，单腔血管球体融合，在体内吻合血管可能涉及类似的机制。Developmental Dynamics 239：398-406，2010. (C)2009 Wiley-Liss，Inc.
We evaluated the self-assembly properties of uniluminal vascular spheroids having outer layers of vascular smooth muscle cells and a contiguous inner layer of endothelial cells lining a central lumen. We showed that while pairs of uniluminal vascular spheroids suspended in culture medium fused to form a larger diameter spheroidal structure, spheroids in collagen hydrogels formed elongated structures. These findings highlight the potential use of uniluminal vascular spheroids as modules to engineer blood vessels. We also demonstrate that uniluminal vascular spheroid fusion conforms to models describing the coalescence of liquid drops. Furthermore, the fusion of uniluminal vascular spheroids in vitro closely resembled the in vivo process by which the descending aorta forms from the fusion of the paired dorsal aortae during embryonic development. Together, the findings indicate that tissue liquidity underlies uniluminal vascular spheroid fusion and that in vivo anastomosis of blood vessels may involve a similar mechanism. Developmental Dynamics 239:398-406, 2010. (C) 2009 Wiley-Liss, Inc.