Metabolic transformation evidence of caffeic acid derivatives in male rats after the oral administration of functional food by UPLC coupled with a hybrid quadrupole-orbitrap mass spectrometer
Metabolic transformation evidence of caffeic acid derivatives in male rats after the oral administration of functional food by UPLC coupled with a hybrid quadrupole-orbitrap mass spectrometer
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UPLC结合混合四极杆轨道阱质谱仪研究雄性大鼠口服功能性食品后咖啡酸衍生物的代谢转化证据
DOI：
10.1039/c4ra15393f
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发表时间：
2015-01-01
期刊：
影响因子：
3.9
通讯作者：
Li, Lei
中科院分区：
文献类型：
--
作者：
Cao, Shuyuan;Zhang, Zhan;Li, Lei
植物性食品或普通饮食中的咖啡酸(CAA)和咖啡酸衍生物(CADS)具有多种生物学功能，这些功能依赖于它们的吸收和代谢。然而，由于功能食品中多组分和微组分并存，其代谢途径和转化机制尚不清楚。本文报道了一种基于超高效液相色谱与Q Exactive混合型四极轨道质谱仪(Q Exactive UPLC-MS/MS)相结合的高效、全面的分析方法，用于同时测定口服典型中国功能食品丹参茶(DSC)后雄性大鼠血浆中的CADS及其代谢物，以及II相酶的转化。从DSC萃取物中共分离鉴定出20种酚酸成分。该方法成功地用于大鼠血浆中15种原型和13种代谢物的同时鉴定。DSC的生物转化是一个II相代谢过程，尤其是硫酸盐化和葡萄糖醛酸化。通过检测大鼠肝脏中硫代转移酶(Sults)和UDP-葡萄糖醛酸基转移酶(UGTS)的mRNA表达，进一步证实了硫化和葡萄糖醛酸化作用。SULT1A1和SULT1B1的诱导作用显著(P<；0.001)。本研究为分析和鉴定复杂生物基质中的DSC及其代谢物提供了一种合适且方便的方法。此外，本研究还揭示了SULTS和UGTS介导的DSC在雄性大鼠体内可能的II相代谢过程。
Caffeic acid (CaA) and caffeic acid derivatives (CADs) in plant-derived foods or common diet have a variety of biological functions, which rely on their absorption and metabolism. However, their metabolic pathways and transformation mechanisms are unclear due to the co-existence of multi- and micro-CADs in functional foods. We report an efficient and comprehensive analytical methodology based on ultra-performance liquid chromatography coupled with a Q Exactive hybrid quadrupole-orbitrap mass spectrometer (Q Exactive UPLC-MS/MS) to simultaneously determine the CADs and their metabolites in male rat plasma after the oral administration of a type of typical Chinese functional food, danshencha (DSC), as well as the transformation of the phase II enzymes. A total of 20 phenolic acid components were obtained and identified from the DSC extracts. This method was successfully applied to the simultaneous identification of 15 prototype and 13 metabolites of CADs in rat plasma. The biotransformation of DSC was a phase II metabolic process, especially sulfation and glucuronidation. The sulfation and glucuronidation were further confirmed by evaluating the mRNA expression of sulfotransferases (SULTs) and UDP-glucoronosyltransferases (UGTs) in rat liver. SULT1A1 and SULT1B1 were induced significantly (P < 0.001). The present study provides a suitable and convenient method to analyze and identify the CADs and metabolites of DSC in complex biological matrices. In addition, this study also reveals the possible phase II metabolic process of DSC mediated by SULTs and UGTs in male rats.