Diclofenac enhances proinflammatory cytokine-induced phagocytosis of cultured microglia via nitric oxide production.
Diclofenac enhances proinflammatory cytokine-induced phagocytosis of cultured microglia via nitric oxide production.
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DOI：
10.1016/j.taap.2013.01.024
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发表时间：
2013-04
影响因子：
3.8
通讯作者：
H. Kakita;Mineyoshi Aoyama;Y. Nagaya;Hayato Asai;M. Hussein;Mieko Suzuki;S. Kato;S. Saitoh;K. Asai
中科院分区：
文献类型：
--
作者：
H. Kakita;Mineyoshi Aoyama;Y. Nagaya;Hayato Asai;M. Hussein;Mieko Suzuki;S. Kato;S. Saitoh;K. Asai
Influenza-associated encephalopathy (IAE) is a central nervous system complication with a high mortality rate, which is increased significantly by the non-steroidal anti-inflammatory drug diclofenac sodium (DCF). In the present study, we investigated the effects of DCF on brain immune cells (i.e. microglia) stimulated with three proinflammatory cytokines, namely tumor necrosis factor-α, interleukin-1β, and interferon-γ. Similar to previous findings in astrocytes, all three cytokines induced the expression of inducible NO synthase (iNOS), as well as NO production, in microglia. The addition of DCF to the culture system augmented iNOS expression and NO production. Immunocytochemical analysis and the phagocytosis assay revealed that cytokine treatment induced morphological changes to and phagocytosis by the microglia. The addition of DCF to the culture system enhanced microglial activation, as well as the phagocytic activity of cytokine-stimulated microglia. Inhibitors of nuclear factor (NF)-κB inhibited iNOS gene expression in cytokine-stimulated microglia with or without DCF, suggesting that the NF-κB pathway is one of the main signaling pathways involved. The iNOS inhibitor NG-monomethyl-l-arginine (l-NMMA) reduced both cytokine-induced phagocytosis and phagocytosis induced by the combination of cytokines plus DCF. Furthermore, the NO donor sodium nitroprusside induced phagocytosis, indicating that NO production is a key regulator of microglial phagocytosis. In conclusion, DCF acts synergistically with proinflammatory cytokines to increase the production of NO in microglia, leading to phagocytic activity of the activated microglia. These findings, together with previous observations regarding astrocytes, may explain the significant increase in mortality of IAE patients treated with DCF.
