SUPPRESSION OF ANTIGEN-INDUCED ARTHRITIS IN LEWIS RATS BY ORAL-ADMINISTRATION OF TYPE-II COLLAGEN
SUPPRESSION OF ANTIGEN-INDUCED ARTHRITIS IN LEWIS RATS BY ORAL-ADMINISTRATION OF TYPE-II COLLAGEN
复制标题
DOI：
10.1002/art.1780380811
复制
发表时间：
1995-08-01
影响因子：
--
通讯作者：
WEINER, HL
中科院分区：
文献类型：
--
作者：
YOSHINO, S;QUATTROCCHI, E;WEINER, HL
目标。为了评估口服II型胶原蛋白（CII）对抗原诱导关节炎（AIA）的作用，方法：在Lewis大鼠中，用Freund完全佐剂中的甲基化牛血清白蛋白（mBSA）免疫诱导关节炎，然后在2周后关节内注射mBSA，不同剂量的CII， mBSA和不相关的对照蛋白，keyhole帽贝血青素（KLH），在诱导关节炎前几天口服5次。测量踝关节肿胀和迟发性超敏反应（DTH）。结果3、30 μ g CII可显著减轻关节肿胀，300 μ g CII无明显作用。10 mg mBSA对AIA的抑制作用最明显，口服KLH无明显作用，mBSA组DTH反应明显降低，CII和KLH组无明显作用。口服CII可以抑制动物模型中的关节炎，其中对胶原蛋白的免疫不起作用，效果是剂量依赖性的，并且在较低剂量的CII下发生。这些结果证明了旁观者抑制与口服耐受性相关的生物学相关性，以及这种方法治疗人类炎症性关节疾病的潜在应用。
Objective. To assess the effect of orally administered type II collagen (CII) in antigen-induced arthritis (AIA),Methods, Arthritis was induced in Lewis rats by immunization with methylated bovine serum albumin (mBSA) in Freund's complete adjuvant, followed by an intraarticular injection of mBSA 2 weeks later, Different doses of CII, mBSA, and an unrelated control protein, keyhole limpet hemocyanin (KLH), were orally administered 5 times over several days prior to the induction of arthritis, Ankle joint swelling and delayed-type hypersensitivity (DTH) responses were measured.Results, Joint swelling was significantly reduced at a dose of 3 mu g and 30 mu g of CII but not at 300 mu g. The most prominent suppression of AIA was observed when rats were fed 10 mg of mBSA, whereas oral KLH had no effect, DTH responses were significantly reduced in the mBSA-fed rats, but not in rats that were fed CII or KLH.Conclusion. Oral CII can suppress arthritis in an animal model in which immunity to collagen does not play a role, The effect is dose dependent and occurs at lower doses of CII. These results demonstrate the biologic relevance of bystander suppression associated with oral tolerance, and the potential use of this approach to treat human inflammatory joint disease.