Enhancement of liver-specific functions of primary rat hepatocytes co-cultured with bone marrow cells on tissue culture-treated polystyrene surfaces.
Enhancement of liver-specific functions of primary rat hepatocytes co-cultured with bone marrow cells on tissue culture-treated polystyrene surfaces.
复制标题
DOI：
10.1007/s10047-005-0289-9
复制
发表时间：
2005-01-01
期刊：
影响因子：
--
通讯作者：
Kawakami, Koei
中科院分区：
文献类型：
--
作者：
Ijima, Hiroyuki;Murakami, Sunao;Kawakami, Koei
将原代大鼠肝细胞与骨髓细胞（BMC）在组织培养处理的培养孔（TCW）或胶原包被的培养孔（CCW）中共培养。虽然使用含血清的培养基，但共培养的细胞在2天内在TCW上形成球状体，并且氨代谢和白蛋白分泌活性良好地维持3周。共培养的细胞在CCW上形成单层2天内，并维持肝功能3周。共培养细胞在CCW上的氨代谢活性和白蛋白分泌活性略低于TCW。这种肝细胞和BMCs的共培养系统是体外表达和维持肝脏特异性功能的合适培养系统，预计可应用于生物人工肝系统、再生医学和肝功能模拟器。
Primary rat hepatocytes were co-cultured with bone marrow cells (BMCs) in a tissue culture-treated culture well (TCW) or a collagen-coated culture well (CCW). Although a medium containing serum was used, the co-cultured cells formed spheroids on the TCW within 2 days, and ammonia metabolism and albumin secretion activities were well maintained for 3 weeks. The co-cultured cells formed a monolayer on the CCW within 2 days, and liver functions were maintained for 3 weeks. The ammonia metabolism activities and albumin secretion activities of co-cultured cells on the CCWs were slightly inferior to those on the TCWs. This co-culture system of hepatocytes and BMCs is an appropriate culture system for the expression and maintenance of liver-specific functions in vitro, and is expected to be applicable to bioartificial liver systems, regenerative medicine, and liver function simulators.