Caenorhabditis elegans embryonic axial patterning requires two recently discovered posterior-group Hox genes.
Caenorhabditis elegans embryonic axial patterning requires two recently discovered posterior-group Hox genes.
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秀丽隐杆线虫胚胎轴向模式需要两个最近发现的后群 Hox 基因。
DOI：
10.1073/pnas.97.9.4499
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发表时间：
2000
影响因子：
11.1
通讯作者：
Wood,WB
中科院分区：
文献类型：
--
作者：
VanAuken,K;Weaver,DC;Edgar,LG;Wood,WB
Hox基因编码高度保守的转录因子，控制区域身份的细胞和组织沿着发展的前后轴，可能在所有的双侧后生动物。然而，在无脊椎动物胚胎thanDrosophila，Hox基因的功能仍然在很大程度上是未知的，除了从序列相似性和表达模式的推断。最近的基因组测序表明，秀丽隐杆线虫具有三个Hox基因，属于后Paraxis组[Ruvkun，G. & Hobert，O.（1998）Science282，2033-2041]。然而，先前仅在遗传上鉴定出一种，并且其不是胚胎发育所需的[奇泽姆，A. 1991年《发展》（英国剑桥）111，921-932]。在此，我们报告了剩余的两个后旁系同源物的鉴定，即nob-1基因和相邻的php-3基因。nob-1和php-3功能的缺失会导致胚胎后部图案形成和后部皮下组织的形态发生运动的总体缺陷，以及从后部到前部的细胞命运转变和致死。胚胎发生所必需的唯一其他Hox基因是thapneial/Hox 1homologceh-13，其是更靠前的模式化所必需的[Brunschwig，K.，Wittmann，C.，施纳贝尔河，Burglin，T. R.，Tobler，H. & Muller，F. 1999年《发展》（英国剑桥）126，1537-1546]。因此，C. elegans只需要Hox基因的前和后paradigs组，提出了有趣的问题，进化的中间组基因。
Hox genes encode highly conserved transcription factors that control regional identities of cells and tissues along the developing anterior–posterior axis, probably in all bilaterian metazoans. However, in invertebrate embryos other thanDrosophila, Hox gene functions remain largely unknown except by inference from sequence similarities and expression patterns. Recent genomic sequencing has shown thatCaenorhabditis eleganshas three Hox genes of the posterior paralog group [Ruvkun, G. & Hobert, O. (1998)Science282, 2033–2041]. However, only one has been previously identified genetically, and it is not required for embryonic development [Chisholm, A. (1991)Development (Cambridge, U.K.)111, 921–932]. Herein, we report identification of the remaining two posterior paralogs as thenob-1gene and the neighboringphp-3gene. Elimination ofnob-1andphp-3functions causes gross embryonic defects in both posterior patterning and morphogenetic movements of the posterior hypodermis, as well as posterior-to-anterior cell fate transformations and lethality. The only other Hox gene essential for embryogenesis is thelabial/Hox1homologceh-13, required for more anterior patterning [Brunschwig, K., Wittmann, C., Schnabel, R., Burglin, T. R., Tobler, H. & Muller, F. (1999)Development (Cambridge, U.K.)126, 1537–1546]. Therefore, essential embryonic patterning inC. elegansrequires only Hox genes of the anterior and posterior paralog groups, raising interesting questions about evolution of the medial-group genes.