SYNTHESIS OF POLYMERIC MICROCAPSULE ARRAYS AND THEIR USE FOR ENZYME IMMOBILIZATION
SYNTHESIS OF POLYMERIC MICROCAPSULE ARRAYS AND THEIR USE FOR ENZYME IMMOBILIZATION
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DOI：
10.1038/369298a0
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发表时间：
1994-05-26
期刊：
影响因子：
64.8
通讯作者：
MARTIN, CR
中科院分区：
文献类型：
--
作者：
PARTHASARATHY, RV;MARTIN, CR
目前用于固定生物反应器和生物传感器 (1-20) 的酶的方法包括吸附或共价连接到支持物 (2-4)、微胶囊化 (5,6) 以及包埋在膜/薄膜 (7,8,11-20) 或凝胶内 (9)。理想的固定化方法应采用温和的化学条件，允许固定大量的酶，在较小的总体积内为酶-底物接触提供大的表面积，最大限度地减少底物和产物质量传输的障碍，并提供化学和机械稳健的系统。在这里，我们描述了一种满足所有这些标准的酶固定化方法。我们开发了一种基于模板的合成方法，可产生直径和长度均一的中空聚合物微胶囊。这些微胶囊排列成高密度阵列，其中各个胶囊像刷子的刷毛一样从表面突出。我们开发了用高浓度酶填充这些微胶囊的程序。负载酶的微胶囊阵列在水溶液和有机溶剂中充当酶生物反应器。
CURRENT methods for immobilizing enzymes for use in bioreactors and biosensors(1-20) include adsorption on or covalent attachment to a support(2-4), micro-encapsulation(5,6), and entrapment within a membrane/film(7,8,11-20) or gel(9). The ideal immobilization method should employ mild chemical conditions, allow for large quantities of enzyme to be immobilized, provide a large surface area for enzyme-substrate contact within a small total volume, minimize barriers to mass transport of substrate and product, and provide a chemically and mechanically robust system. Here we describe a method for enzyme immobilization that satisfies all of these criteria. We have developed a template-based synthetic method that yields hollow polymeric microcapsules of uniform diameter and length. These microcapsules are arranged in a high-density array in which the individual capsules protrude from a surface like the bristles of a brush. We have developed procedures for filling these microcapsules with high concentrations of enzymes. The enzyme-loaded microcapsule arrays function as enzymatic bioreactors in both aqueous solution and organic solvents.