High-Throughput Profiling of Caenorhabditis elegans Starvation-Responsive microRNAs.
High-Throughput Profiling of Caenorhabditis elegans Starvation-Responsive microRNAs.
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秀丽隐杆线虫饥饿响应性microRNA的高通量分析。
DOI：
10.1371/journal.pone.0142262
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Miranda-Rios J
中科院分区：
文献类型：
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作者：
Garcia-Segura L;Abreu-Goodger C;Hernandez-Mendoza A;Dimitrova Dinkova TD;Padilla-Noriega L;Perez-Andrade ME;Miranda-Rios J
MicroRNAs （miRNAs）是一种长度约为22个核苷酸的非编码rna，通过干扰mrna的稳定性和翻译来调节基因表达。它们的表达在发育过程中，在各种应激条件下和几个病理过程中受到调节。在自然界中，动物经常面临盛宴或饥荒的情况。我们观察到，将秀丽隐杆线虫的早期L4幼虫进行12小时的饥饿期，产生的蠕虫比喂养良好的动物更瘦，更短，脂肪积累减少，后代减少，性腺大小缩小，寿命延长。我们的目的是通过对早期L4幼虫的深度测序，确定在饥饿条件下秀丽隐杆线虫的302种已知mirna中，哪些mirna的表达与喂养良好的线虫相比发生了变化。我们的研究结果表明，在饥饿12小时与良好喂养的早期L4幼虫中，13种mirna （miR-34-3p， miR-35-3p至miR-41-3p， miR-39-5p, miR-41-5p, miR-240-5p， miR-246-3p和miR-4813-5p）上调，而2种mirna （let-7-3p和miR-85-5p）下调。在饥饿条件下改变表达的mirna的一些预测目标涉及代谢或发育过程。特别是，miR-35家族的mirna在饥饿时上调6-20倍。此外，我们发现，当miR-35-3p的表达上调时，在卵子发生中重要的gld-1 （miR-35-3p的验证靶点）的表达下调。另一个已报道的靶标细胞周期调节因子lin-23的表达在饥饿期间没有变化。这项研究为更全面地了解秀丽隐杆线虫饥饿过程中mirna的作用提供了一个起点。
MicroRNAs (miRNAs) are non-coding RNAs of ~22 nucleotides in length that regulate gene expression by interfering with the stability and translation of mRNAs. Their expression is regulated during development, under a wide variety of stress conditions and in several pathological processes. In nature, animals often face feast or famine conditions. We observed that subjecting early L4 larvae from Caenorhabditis elegans to a 12-hr starvation period produced worms that are thinner and shorter than well-fed animals, with a decreased lipid accumulation, diminished progeny, reduced gonad size, and an increased lifespan. Our objective was to identify which of the 302 known miRNAs of C. elegans changed their expression under starvation conditions as compared to well-fed worms by means of deep sequencing in early L4 larvae. Our results indicate that 13 miRNAs (miR-34-3p, the family of miR-35-3p to miR-41-3p, miR-39-5p, miR-41-5p, miR-240-5p, miR-246-3p and miR-4813-5p) were upregulated, while 2 miRNAs (let-7-3p and miR-85-5p) were downregulated in 12-hr starved vs. well-fed early L4 larvae. Some of the predicted targets of the miRNAs that changed their expression in starvation conditions are involved in metabolic or developmental process. In particular, miRNAs of the miR-35 family were upregulated 6–20 fold upon starvation. Additionally, we showed that the expression of gld-1, important in oogenesis, a validated target of miR-35-3p, was downregulated when the expression of miR-35-3p was upregulated. The expression of another reported target, the cell cycle regulator lin-23, was unchanged during starvation. This study represents a starting point for a more comprehensive understanding of the role of miRNAs during starvation in C. elegans.