AN EXPERIMENTAL ANALYSIS OF COMB DEVELOPMENT IN CHICKEN
AN EXPERIMENTAL ANALYSIS OF COMB DEVELOPMENT IN CHICKEN
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DOI：
10.1002/jez.1401520209
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发表时间：
1963-01-01
影响因子：
--
通讯作者：
LAWRENCE, IE
中科院分区：
文献类型：
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作者：
LAWRENCE, IE
本文对鸡冠发育进行了实验分析。由于原基阐述了一个独特的脊修改真皮，拥有一个薄的表皮，和表型遗传的梳模式是特定的，标准的实验胚胎学技术是适用的。新罕布什尔州红鸭（单冠）、毛茛鸭（双冠）和白色北京鸭（无冠）组成实验材料。本研究采用三种方法：（1）比较单冠和双冠组织的发育，（2）睾酮对发育器官的影响，（3）绒毛尿囊移植物中的组织组合。在这项调查中获得了以下结果：在单和双梳型，一个特征性的海拔，从中胚层冷凝对应的表型模式，发生在第6和第7天的孵育。组织学细节遵循早期作者的描述。肥大的梳子组织产生通过应用睾酮没有观察到的结构修改。根据绒毛膜-尿囊移植物中梳状细胞的分化能力，最早确定原基的时间为Hamburger-Hamilton分期24（4天）。制备了10种不同的前瞻性和非前瞻性组织组合，在绒毛膜-尿囊移植物中纳入同种、异种和异种组合。假定梳状中胚层的新罕布什尔州红色分化的中胚层供体的单梳特征，当与非梳状外胚层的新罕布什尔州红色，假定梳状外胚层的毛茛，非梳状外胚层的毛茛，和前额外胚层的鸭。毛茛假定梳状中胚层发育了一种双重结构，这是毛茛品种的特征，与毛茛的非梳状外胚层、新罕布什尔州红的假定梳状外胚层和新罕布什尔州红的非梳状外胚层相关。假定的梳子外胚层的新罕布什尔州红色也没有毛茛产生一个可识别的梳子时，结合中胚层从一些非梳子来源，包括前额中胚层的鸭。外胚层从推定的羽毛道没有发展羽毛乳头时，纳入梳子。
An experimental analysis of development in the chicken comb has been investigated. Since the primordium elaborates a distinct ridge of modified dermis, possessing a thin epidermis, and phenotypic inheritance of comb pattern is specific, standard experimental embryological techniques were applicable. The New Hampshire Red (single comb), the Buttercup (duplex comb), and the White Pekin duck (non-comb) comprised the experimental material. Three approaches were made in the study: (1) a comparison of single and duplex comb tissues in development, (2) the influence of testosterone on the developing organ, and (3) tissue combinations in chorio-allantoic grafts. The following results were obtained in this investigation: In both single and duplex comb types, a characteristic elevation, resulting from mesodermal condensation corresponding to the phenotypic pattern, occurred between the 6th and 7th day of incubation. The histological details followed those of earlier authors. Hypertrophy of the comb tissues was produced through an application of testosterone with no observable structural modifications. The earliest determination of the primordium based upon the ability to differentiate comb in chorio-allantoic grafts was Hamburger-Hamilton stage 24 (4 days). Ten different combinations of prospective and non-prospective tissues were made, incorporating homoplastic, heteroplastic and xenoplastic combinations in chorio-allantoic grafts. Presumptive comb mesoderm of the New Hampshire Red differentiated a single comb characteristic of the mesodermal donor, when combined with non-comb ectoderm of the New Hampshire Red, the presumptive comb ectoderm of the Buttercup, non-comb ectoderm of the Buttercup, and forehead ectoderm of the duck. The Buttercup presumptive comb mesoderm developed a duplex structure, characteristic of the Buttercup variety, in association with non-comb ectoderm of the Buttercup, presumptive comb ectoderm of the New Hampshire Red, and non-comb ectoderm of the New Hampshire Red. Presumptive comb ectoderm of neither the New Hampshire Red nor the Buttercup produced a recognizable comb when combined with mesoderm from a number of non-comb sources including forehead mesoderm of the duck. Ectoderm from presumptive feather tracts did not develop feather papillae when incorporated into a comb.